Folin ciocalteu

Folin–Ciocalteu and gallic acid-1-hydrate (C₆H₂(OH)₃COOH·H₂O) were from PanReac (Cascais, Portugal). Quercetin dihydrate (C₁₅H₁₀O₇·2H₂O) was from Extrasynthese (Lyon, France). Anhydrous aluminum chloride, potassium acetate, sodium carbonate, and Tris(hydroxymethyl)amino methane were from Merck (Alges, Portugal). Phenazine methosulfate (PMS), nicotinamide-adenine dinucleotide hydride (NADH), and nitro-blue tetrazolium chloride (NBT) were from Sigma Aldrich (Lisbon, Portugal). All reagents were pro-analysis grade. All absorbance measurements were performed in a Perkin–Elmer (Lisbon, Portugal) Lambda 25. The reagents were weighed on an analytical balance (Sartorius, ±0.00001 g) (Lisbon, Portugal).

Folin–Ciocalteu and gallic acid-1-hydrate (C6H2(OH)3COOH.H2O) were from PanReac (Castellar del Vallès, Barcelona); tannic acid, TPTZ 2,4,6-tri (2-pyridyl)-s-triazine, Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2′-azino-bis (3-ethylbenzo thiazoline-6-sulfonic acid), potassium persulfate (K2S2O8), NADH (nicotine-adenine dinucleotide reduced), NBT (nitro blue tetrazolium) ≥97%, and PMS (phenazine methosulfate) were from Sigma-Aldrich (Buenos Aires, Argentina); 1-butanol, NED (1- naphthyl ethylenediamine dihydrochloride), and sulfanilic acid were from Merck (Darmstadt, Germany); procyanidin A2 was from Extrasynthese (Genay, França); potassium iodate was from VWR Analar Normapur; and sodium nitroprusside was from Riedel-de Haën (Charlotte, NC, USA). All reagents were pro analysis grade. All absorbance measurements were performed in a Perkin-Elmer Lambda 25 (Watertown, MA, USA). The reagents were weighed on an analytical balance (Sartorius ± 0.0001 g).