The Enzyme Commission (EC) number of SERCA is 7.2.2.10 3 . The EC of GP is 2.4.1.1 . Two enzymes for the linked-enzyme ATPase assay – pyruvate kinase (EC 2.7.1.40 ) and L-lactate dehydrogenase (EC 1.1.1.27 ) – were purchased from Sigma-Aldrich Corp. Standard chemicals and Stains-all dye were purchased from Sigma-Aldrich. Laemmli-type SDS-PAGE gels were purchased from Bio-Rad Laboratories, Inc. The BCA Protein Assay Kit was purchased from Pierce Biotechnology, Inc. Primary and secondary antibodies for immunoblotting were purchased from specific companies, as described below.
Laemmli type sds page gels
Manufactured by Bio-Rad
Laemmli-type SDS-PAGE gels are laboratory equipment used for the separation and analysis of proteins based on their molecular weight. They provide a standardized method for protein electrophoresis, allowing for the effective separation and visualization of complex protein mixtures.
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2 protocols using laemmli type sds page gels
1
Linked-Enzyme ATPase Activity Assay
2
Synthesis and Characterization of SLN Peptides
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Horse SLN (29 residues), rabbit SLN (31 residues), and human SLN (31 residues) peptides were synthesized by New England Peptides, Inc., using Fmoc solid-phase chemistry at 50-mg crude scale (16 µmol). Quality assessment of peptide synthesis was validated using high-performance liquid chromatography (HPLC) and mass spectrometry. The three SLN peptides were synthesized with an acetylated N-terminus and an amidated C-terminus. Synthetic SLN peptides were further purified in-house by HPLC [65 (link)]. The concentration of synthetic SLN peptides was determined by amino acid analysis, BCA assay, and densitometry of Coomassie blue-stained gels [34 (link),66 (link)]. Laemmli-type SDS-PAGE gels were purchased from Bio-Rad Laboratories, Inc.
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