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Fabp1

Manufactured by Abcam
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FABP1 is a protein that is involved in the intracellular transport of fatty acids. It belongs to the fatty acid-binding protein family.

Automatically generated - may contain errors

Lab products found in correlation

Pparγ, by Abcam (2 mentions) Envision plus kit, by Agilent Technologies (2 mentions) C fos, by Santa Cruz Biotechnology (1 mentions) Vinculin, by Merck Group (1 mentions) Fra 1, by Santa Cruz Biotechnology (1 mentions) C ebpα, by Santa Cruz Biotechnology (1 mentions) Gotaq qpcr master mix, by Eppendorf (1 mentions) Fsp27, by Novus Biologicals (1 mentions) Pparγ, by Santa Cruz Biotechnology (1 mentions) Total creb, by Cell Signaling Technology (1 mentions) Pparγ, by Cell Signaling Technology (1 mentions) C ebpβ, by Cell Signaling Technology (1 mentions) C jun, by Cell Signaling Technology (1 mentions) Parp 1, by Santa Cruz Biotechnology (1 mentions)

3 protocols using fabp1

1

Immunohistochemical Staining of Tissue Microarrays

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Table 1 contains a summary of the immunohistochemical stains used. Briefly, stains for each antigen were performed on 5 μm paraffin sections of each tissue microarray masterblock. The panel included antibodies against CD3 (rabbit polyclonal, RTU, Dako, Carpinteria, CA), FABP1 (mouse monoclonal, 1:1000, Abcam, Cambridge, MA), and PPARγ (rabbit polyclonal, 1:50, Abcam). The stains were run on the Dako autostainer using the Envision Plus kit (Dako). Slides were counterstained with hematoxylin, dehydrated and coverslipped. Positive controls included normal colon (PPAR), normal tonsil (CD3), and normal liver (FABP).
Wood S.M., Gill A.J., Brodsky A.S., Lu S., Friedman K., Karashchuk G., Lombardo K., Yang D, & Resnick M.B. (2016). Fatty acid binding protein 1 is preferentially lost in microsatellite instable colorectal carcinomas and is immune modulated via the interferon γ pathway. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 30(1), 123-133.
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2

Transcriptional Regulation of Adipogenesis

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qRT-PCR was performed using the GoTaq® qPCR Master Mix and an Eppendorf light cycler. Expression levels were calculated using the ΔCt-method. Data were normalized to a housekeeping gene (rps27 or rpl0). Primer sequences are available upon request. Immunoblot analysis was performed using standard protocols and following antibodies: ACC, PPARγ, CEBPβ, c-Jun, phospho-CREBP, total CREB (Cell Signaling), Vinculin (Sigma), PPARγ, Parp-1, CEBPα, c-Fos, Fra-1 (Santa Cruz), HNF4, FAS, Fabp1 (Abcam), and Fsp27 (Novus Biologicals), SREBP-1/2 (BD Bioscience). Nuclear extracts from liver tissue were obtained using the NE-PER Nuclear Protein Extraction Kit (Pierce).
Hasenfuss S.C., Bakiri L., Thomsen M.K., Williams E.G., Auwerx J, & Wagner E.F. (2014). Regulation of steatohepatitis and PPARγ signaling by distinct AP-1 dimers. Cell metabolism, 19(1), 84-95.
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3

Immunohistochemical Staining of Tissue Microarrays

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Table 1 contains a summary of the immunohistochemical stains used. Briefly, stains for each antigen were performed on 5 μm paraffin sections of each tissue microarray masterblock. The panel included antibodies against CD3 (rabbit polyclonal, RTU, Dako, Carpinteria, CA), FABP1 (mouse monoclonal, 1:1000, Abcam, Cambridge, MA), and PPARγ (rabbit polyclonal, 1:50, Abcam). The stains were run on the Dako autostainer using the Envision Plus kit (Dako). Slides were counterstained with hematoxylin, dehydrated and coverslipped. Positive controls included normal colon (PPAR), normal tonsil (CD3), and normal liver (FABP).
Wood S.M., Gill A.J., Brodsky A.S., Lu S., Friedman K., Karashchuk G., Lombardo K., Yang D, & Resnick M.B. (2016). Fatty acid binding protein 1 is preferentially lost in microsatellite instable colorectal carcinomas and is immune modulated via the interferon γ pathway. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 30(1), 123-133.
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