Expression of stem cell surface markers in DPCs was determined by fluorescence-activated cell sorting (FACS) analysis. The cells were detached using trypsin in 0.25% elhylene diamine tetraacetic acid (EDTA). After neutralization, single-cell suspensions were washed with phosphate-buffered saline supplemented with 2% FBS and 0.01% NaN3 (FACS buffer). Quantities of 1 × 105 cells were incubated with the conjugated antibody for 20 min on ice in the dark. After washing, fluorescence intensity was measured on FACS Aria II cell sorter (BD Biosciences, San Jose, CA, USA). The following antibodies were used: Phycoerythrin (PE)-CD271 (Miltenyi Biotec, Auburn, CA, USA), Fluorescein Isothiocyanate (FITC)-CD90 (Biolegend, San Diego, CA, USA), allophycocyanin (APC)-CD106 (Biolegend, San Diego, CA, USA) or dual color combinations APC-STRO-1/PE-CD146 (Both from: Biolegend, San Diego, CA, USA), and PE-CD51 (Biolegend, San Diego, CA, USA)/APC-CD140α (BD Biosciences, San Jose, CA, USA). PE-IgG was used as a negative control.
Fluorescein isothiocyanate fitc cd90
Manufactured by BioLegend
Sourced in United States
Fluorescein Isothiocyanate (FITC)-CD90 is a fluorescently labeled antibody that binds to the CD90 (Thy-1) cell surface antigen. CD90 is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on various cell types, including hematopoietic stem cells, mesenchymal stem cells, and certain subsets of T cells.
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2 protocols using fluorescein isothiocyanate fitc cd90
1
Flow Cytometry Analysis of Stem Cell Markers
2
Stem Cell Surface Marker Expression Analysis
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Expression of stem cell surface markers in PDLCs was determined by fluorescent activated cell sorting (FACS) analysis. The cells were detached using trypsin in 0.25% ethylenediaminetetraacetic acid (EDTA). After neutralization, single-cell suspensions were washed with phosphate-buffered saline (PBS) supplemented with 2% FBS and 0.01% NaN3 (FACS buffer). Quantities of 1 × 106 cells were incubated with direct conjugated antibodies for 20 min on ice in the dark. After washing, fluorescence intensity was measured on FACS Aria II cell sorter (BD Biosciences, San Jose, CA, USA). The following anti-human antibodies were used: phycoerythrin (PE)-CD271 (Miltenyi Biotec, Auburn, CA, USA), fluorescein isothiocyanate (FITC)-CD90 (BioLegend, San Diego, CA, USA), allophycocyanin (APC)-CD106 (BioLegend, San Diego, CA, USA) or dual color combinations APC-STRO-1/PE-CD146 (Both from: BioLegend, San Diego, CA, USA), and PE-CD51 (BioLegend, San Diego, CA, USA)/APC-CD140α (BD Biosciences, San Jose, CA, USA). PE-IgG was used as a negative control.
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