Hrp conjugated donkey anti rabbit and sheep anti mouse secondary antibodies

Antibodies were from the following sources: monoclonal anti-PP2A_Cα/β (05-421), monoclonal anti-methyl PP2A_c (2A10) and monoclonal anti-demethylated PP2A_C (4B7) antibodies were purchased from Millipore/Upstate Biotechnologies, UK; polyclonal anti-Gα_t1 (K20), monoclonal anti-LCMT-1 (4A4), polyclonal anti-PP2A_C (FL-309) and monoclonal anti-PME-1 (B12) antibodies were purchased from Santa Cruz Biotechnology; polyclonal anti-cardiac troponin I (cTnI), monoclonal anti-PKB (2H10), rabbit monoclonal anti-PKB (11E7) and phosphorylated PKB (ser473) were obtained from Cell Signaling Technology. Polyclonal anti-GFP antibody was purchased from Clontech; anti-B55α (PPP2R2A) polyclonal antibody was purchased from Merck Calbiochem; HRP-conjugated donkey anti-rabbit and sheep anti-mouse secondary antibodies were purchased from GE Healthcare, UK. G_iPCR agonists N⁶-cyclopentyladenosine (CPA), carbachol (CCH) and CGP42112 (CGP) were purchased from Sigma-Aldrich; LY294002 was purchased from Merck Calbiochem. Recombinant adenovirus coexpressing enhanced green fluorescent protein (EGFP) and the Gα subunit of transducin (Gα_t1) was a kind gift from Professor Thomas Wieland [27] (link), University of Heidelberg, Germany.

Antibodies were purchased as indicated: β1 integrin (BD Biosciences), EGFR, Erk1/2, p-Erk1/2 (T202/Y204), FAK, p-FAK (Y397), mTOR, p-mTOR (S2448) (Cell Signaling); KEAP1 (GeneTex), β-Actin (Sigma), p-EGFR (Y1068) (Thermo Fisher Scientific); HRP-conjugated donkey anti-rabbit and sheep anti-mouse secondary antibodies (GE Healthcare).