Column chromatography (CC), lichroperp RP-18 25-40 μm; thin layer chromatography (TLC), silica gel GF254 plates (20 × 20 cm, 0.5 mm (Merck, Germany); screening by spattering with cerium sulphate (Sigma, USA) in 10% aq. H2SO4 and using heat. All solvents used were of high pressure liquid chromatography (HPLC) grade (Caledon Laboratory Chemicals Ltd. Georgetown, Ont. Canada). Neuclear magnetic resonance (NMR; Bruker, USA) AV-400 (1H) and AV-100 (13C); EI-MS: waters Q-TOF Micro YA019 mass spectrometer (USA).
Cerium sulphate
Manufactured by Merck Group
Sourced in United States
Cerium sulfate is a chemical compound with the formula Ce(SO4)2. It is a pale yellow-white crystalline solid that is used as a laboratory reagent and oxidizing agent.
Automatically generated - may contain errors
Lab products found in correlation
Gf254 plates, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Avantor (1 mentions)
Milli q50 purification system, by Merck Group (1 mentions)
Deuterated methanol cd3od, by Merck Group (1 mentions)
Hplc grade methanol, by Merck Group (1 mentions)
Mtt reagent, by Merck Group (1 mentions)
Lc 8a series pumping system, by Shimadzu (1 mentions)
Avance 250, by Bruker (1 mentions)
Lcq advantage thermofinnigan spectrometer, by Thermo Fisher Scientific (1 mentions)
C 18 bondapak column, by Waters Corporation (1 mentions)
Ap 300, by Atago (1 mentions)
Q tof premier mass spectrometer, by Waters Corporation (1 mentions)
Rid 10a, by Shimadzu (1 mentions)
Drx 500, by Bruker (1 mentions)
3 protocols using cerium sulphate
1
Purification and Characterization of Compounds
2
Analytical Solvent Extraction and Chromatography
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Analytical-grade solvents, including n-hexane, chloroform, and methanol, were used for the extraction and isolation procedures. The MTT reagent (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), HPLC-grade methanol, and deuterated methanol (CD3OD) were purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany). Cerium sulphate, used in the study, was also obtained from Sigma-Aldrich. HPLC-grade water with a resistivity of 18 MΩ was prepared using a Milli-Q50 purification system from Millipore Corp. (Bedford, MA, USA). The Sephadex LH-20 resin employed in the chromatography procedure was purchased from Supelco (Merck KGaA, Darmstadt, Germany). Furthermore, dimethyl sulfoxide (DMSO), used for solubilizing the extract and molecules during bioassays, was obtained from VWR Chemicals (VWR International Srl, Milano, Italy).
3
Analytical Techniques for Natural Product Characterization
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Optical rotations were measured on an Atago AP-300 digital polarimeter equipped with a sodium lamp (589 nm) and a 1 dm microcell. 1D and 2D NMR experiments were recorded at 300 K in CD 3 OD on a Bruker DRX-500 and Bruker Avance 250 spectrometers (Bruker BioSpin GmBH) [30] . HRESIMS were acquired in the positive ion mode on a Q-TOF premier mass spectrometer (Waters). ESI-MS spectra were obtained from an LCQ Advantage ThermoFinnigan spectrometer (ThermoFinnigan), equipped with Xcalibur software. Column chromatography was carried out over Sephadex LH-20 (40-70 µm, Pharmacia). High-Performance Centrifugal Partition Chromatography (HPCPC) was performed on an Everseiko CPC240 instrument equipped with 3136 cells (total volume, 240 mL). HPLC separations were conducted on a Shimadzu LC-8A series pumping system equipped with a Shimadzu RID-10A refractive index detector and Shimadzu injector on a C18 µ-Bondapak column (30 cm x 7.8 mm, 10 µm Waters, flow rate 2.0 mL/min). TLC separations were carried out using silica gel 60 F 254 (0.20 mm thickness) plates (Merck) and cerium sulphate (Sigma-Aldrich) as spray reagent.
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