Antibodies against GRP78, CHOP, and α-SMA were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibody against KIM-1 (kidney injury molecule-1) was purchased from R&D Systems (Minneapolis, MN, USA). Antibodies against GAPDH were purchased from Proteintech (Chicago, IL, USA). Antibodies against ATF-4 were purchased from Bioss (Beijing, China). Antibodies against β-tubulin were purchased from Elabscience (Wuhan, China). Antibody against Fibronectin 1 (FN1) were purchased from ZEN-BIOSCIENCE (Chengdu, China). Secondary antibodies and 4′,6-diamidino-2-phenylindole (DAPI) were purchased from Invitrogen (Carlsbad, CA, USA). N-acetylcysteine (NAC) was purchased from Yeasen (Shanghai, China). tert-Butyl hydroperoxide (TBHP) was purchased from Macklin (Shanghai, China). Fetal bovine serum (FBS) was purchased from Suero fetal bovine ester, Natocor-Industria Biologica (Cordoba, Argentina). Bovine serum albumin (BSA) was purchased from Biosharp (Anhui, China). Triton X-100 was purchased from SolarBio (Beijing, China). Radioimmunoprecipitation assay (RIPA) buffer was purchased from Epizyme (Shanghai, China). BeyoECL Plus reagent was purchased from Beyotime Biotechnology (Shanghai, China).
N acetylcysteine nac
Manufactured by Yeasen
Sourced in China
N-acetylcysteine (NAC) is a naturally occurring compound that serves as a precursor to the antioxidant glutathione. It is commonly used in various laboratory applications and research settings.
Automatically generated - may contain errors
Lab products found in correlation
Antibodies against grp78, by Cell Signaling Technology (1 mentions)
Tert butyl hydroperoxide tbhp, by Maclin Biochemical Technology (1 mentions)
Antibodies against gapdh, by Proteintech (1 mentions)
Bovine serum albumin bsa, by Biosharp (1 mentions)
α sma, by Cell Signaling Technology (1 mentions)
Beyoecl plus reagent, by Beyotime (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Triton x 100, by Solarbio (1 mentions)
Au680 clinical chemistry analyzer, by Beckman Coulter (1 mentions)
2 protocols using n acetylcysteine nac
1
Comprehensive Antibody Sourcing for Cellular Analyses
2
Partial Hepatic Warm Ischemia-Reperfusion Injury Model
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A mature model of partial hepatic warm IRI has been used [12 (link), 33 (link)]. Briefly, after successful anesthesia with isoflurane, heparin was injected into the mice. A midline laparotomy was performed, and an atraumatic clip was used to interrupt the arterial and portal venous blood supply to the cephalic lobes (70%) of the liver. After 90 min of partial hepatic warm ischemia, the clip was removed to initiate the process of hepatic reperfusion. Mice were maintained anesthetized with isoflurane and placed in the environment temperature at 26 °C. The mice were killed after 6 h of reperfusion and the collected samples were harvested for analysis. Sham controls underwent the same procedure but without vascular occlusion.
To study the effects of ROS, mice were pretreated with 300 mg/kg N-acetylcysteine (NAC) (Yeasen Biotechnology, Shanghai, China) or PBS by intraperitoneal injection.
Serum ALT and AST levels were measured using an AU680 clinical chemistry analyzer (Beckman Coulter, Brea, California, USA).
Liver specimens were fixed in 4% paraformaldehyde and embedded in paraffin for hematoxylin and eosin (H&E) and immunofluorescent staining. Some of the specimens were frozen in liquid nitrogen for 8-OHdG staining.
To study the effects of ROS, mice were pretreated with 300 mg/kg N-acetylcysteine (NAC) (Yeasen Biotechnology, Shanghai, China) or PBS by intraperitoneal injection.
Serum ALT and AST levels were measured using an AU680 clinical chemistry analyzer (Beckman Coulter, Brea, California, USA).
Liver specimens were fixed in 4% paraformaldehyde and embedded in paraffin for hematoxylin and eosin (H&E) and immunofluorescent staining. Some of the specimens were frozen in liquid nitrogen for 8-OHdG staining.
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