K18 ace2 2prlmn j

Animal studies were carried out in accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. The protocols were approved by the Institutional Animal Care and Use Committee at the Washington University School of Medicine (assurance number A3381–01). Virus inoculations were performed under anesthesia that was induced and maintained with ketamine hydrochloride and xylazine, and all efforts were made to minimize animal suffering.
Heterozygous K18-hACE2 C57BL/6J mice (strain: 2B6.Cg-Tg(K18-ACE2)2Prlmn/J, Cat # 34860) were obtained from The Jackson Laboratory. Animals were housed in groups and fed standard chow diets.
Eight-week-old female K18-hACE2 C57BL/6 mice were administered 100 μg of 2130-1-0114-112, parental 2130, or isotype control anti-West Nile virus hE16 mAb^{61 (link)} by intraperitoneal injection one day before intranasal inoculation with 104 focus-forming units (FFU) of WA1/2020 D614G, BA.1.1 or BA.5. Animals were euthanized at 4 days post-infection and tissues were harvested for virological analysis.

Animal housing and experimental procedures have been conducted according to the French and European Regulations (Parlement Européen et du Conseil du 22 Septembre 2010. Décret n°2013-118 du 1er Février 2013 relatif à la protection des animaux utilisés à des fins scientifiques) and the National Research Council (U.S.), Institute for Laboratory Animal Research (U.S.), and National Academies Press (U.S.), Eds., Guide for the care of laboratory animals, 8th ed. Washington, D.C: National Academies Press, 2011. The CIPHE BSL3 facility operates under Agreement N° B 13 014 07 delivered by the French authorities. All animal procedures (including surgery, anesthesia and euthanasia as applicable) used in the current study have been submitted to the Institutional Animal Care and Use Committee of CIPHE approved by French authorities (CETEA DSV -APAFIS#26484-2020062213431976 v6). All the CIPHE BSL3 facility operations are oversee by a Biosecurity/Biosafety Officer and accredited by Agence Nationale de Sécurité du Médicament (ANSM). K18-hACE C57BL/6J mice (strain: 2B6.Cg-Tg (K18-ACE2)2Prlmn/J) were obtained from The Jackson Laboratory. Animals were housed in groups and fed standard chow diets.