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Goat polyclonal ab

Manufactured by R&D Systems
Sourced in United States

Goat polyclonal antibodies are immunoglobulin proteins produced by goats in response to an antigen. They are a mixture of antibodies that recognize multiple epitopes on the target antigen.

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2 protocols using goat polyclonal ab

1

Immunohistochemical and Immunofluorescence Analysis of Immune Markers

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The following antibodies (Abs) were used for immunohistochemical staining: mouse monoclonal Abs (LifeSpan BioScience, Seattle, WA, United States) against human CCL20 and human IL-36γ, rabbit polyclonal Abs (LifeSpan BioScience) against human CYP1A1 and human IL-23, and a goat polyclonal Ab (R&D Systems, Minneapolis, MN, United States) against human IL-17. The following antibodies were used for immunofluorescence (IF): mouse anti-human CD163 phycoerythrin-conjugated monoclonal antibody (R&D Systems), rabbit polyclonal anti-CCL22 antibody (R&D Systems), rabbit polyclonal anti-CXCL5 antibody (Lifespan Bioscience, Seattle, WA, United States), mouse anti-CXCL10 antibody (Lifespan Bioscience), Alexa Fluor 488-conjugated anti-mouse rat immunoglobulin (Ig)G (Abcam, Tokyo, Japan), and Alexa Fluor 488-conjugated anti-rabbit goat IgG (Abcam).
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2

Immunohistochemical Profiling of Tumor Specimens

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Serial 4 μm FFPE pre-treatment tumor specimens from patients receiving anti-PD-1 were stained for CD8 (n = 23/26, mAb clone 144B, Dako, Carpinteria, CA), PD-1 (n = 16/26, goat polyclonal Ab, R&D Systems, Minneapolis, MN) or PD-L1 (n = 25/26, mAb clone 22C3, Merck Research Laboratories, White House Station, NJ) by IHC/IF, as previously described [11 (link)]. Archival specimens from patients not treated with anti-PD-1 were stained for NKp46 using IHC. Appropriate positive and negative controls for each marker were run with every batch.
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