Dimethyl sulfoxide (dmso)

Extract of ginger dried root (batch number ZSKY20140123) was purchased from Shaanxi Zhengsheng Kangyuan Bio-medical Co., Ltd (Shaanxi, China). Detailed information about this extract is summarized in Table 1a. Dexamethasone was bought from caspiantamin (Rasht, Iran). Dimethyl-sulfoxide (DMSO) was obtained from Cinnagen (Tehran, Iran). Roswell Park Memorial Institute-1640 (RPMI1640), fetal bovine serum (FBS), and penicillin streptomycin (Pen Strep) were purchased from Bioidea (Tehran, Iran). l-Glutamine was from Gibco (Sao Paulo, Brazil) and phosphate buffered saline (PBS) was bought from Sigma-Aldrich (Munich, Germany). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was obtained from Atocel (Graz, Austria). FITC Annexin-V apoptosis detection kit with PI was purchased from BioLegend (London, United Kingdom). TRIzol reagent was from Invitrogen (California, CA) and Ficoll–Hypaque was bought from Inno-train (Kronberg, Germany).

Ginger extract was obtained from Shaanxi Zhengsheng Kangyuan Bio‐medical Co., Ltd. 6‐Shogaol was purchased from Adooq Bioscience. 10‐gingerol, phosphate‐buffered saline (PBS), mouse anti‐p21 monoclonal antibody, 2′,7′‐Dichloro‐fluorescin diacetate (DCFH‐DA) and N‐acetyl‐l‐cysteine (NAC) were bought from Sigma‐Aldrich. MTX, mouse anti‐p53 monoclonal antibody and mouse anti‐β actin monoclonal antibody were acquired from Santa Cruz Biotechnology, Inc. Dimethyl‐sulfoxide (DMSO) was from Cinnagen. Roswell Park Memorial Institute‐1640 (RPMI1640), foetal bovine serum (FBS) and penicillin streptomycin (Pen Strep) were obtained from Bioidea. 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) was from Atocel. l‐glutamine was bought from Gibco and FITC Annexin‐V Apoptosis Detection Kit with PI was purchased from BioLegend. Ficoll–Hypaque was obtained from Inno‐train and TRIzol reagent was from Invitrogen. PrimeScript™ RT reagent Kit was from Takara. Protein ladder and goat anti‐mouse immunoglobulins/HRP were from Thermo Fisher Scientific and Dako Denmark A/S respectively. Nitrocellulose membrane and Amersham ECL Prime Western Blotting Detection Reagent were purchased from GE Healthcare.