Jeko 1

The human MCL cell lines JeKo-1, JeKo BTK KD, Mino, Mino ABT-199 R, Rec-1, Rec ABT-199 R, and Maver-1 cells were maintained within RPMI 1640 medium supplemented with 1% penicillin/streptomycin, 25 mM 4-(2-hydroxyethyl)-1piperazineethanesulfonic acid (HEPES), and 10% fetal bovine serum (FBS; Sigma-Aldrich, St Louis, MO). These cells were cultured in a CO₂ incubator at 37 °C. The MCL cell lines Rec-1, JeKo-1, Z-138, Maver-1, JVM-2, and JVM-13 were obtained from the American Type Culture Collection (ATCC). The Mino cell line was originally established and provided by Dr. Richard Ford at MD Anderson Cancer Center. The JeKo-BTK KD cell line was generated by the MD Anderson Core Facility and previously verified and published^{65 (link)}. Venetoclax (ABT-199)-resistant MCL cell lines (Mino-ABT-199 R and Rec-1 ABT-199 R) were generated from the parental cell lines (Mino and Rec-1) by multistep exposures of cells to increasing doses (up to 100 nM) of venetoclax for 8 weeks as previously described^{66 (link)}.

Jeko-1, Mino, MM.1S, and HEK293T cells were bought from the American Type Culture Collection (ATCC), and DAOY cells were a gift from Dr. Alex Huang (Case Western Reserve University, OH, USA). Jeko-1, Mino, MM.1S, and DAOY cells were cultured in RPMI-1640 (Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich) and 1% Pen/Strep (Hyclone). HEK293T cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) (Sigma-Aldrich) with the same 10% FBS and 1% Pen/Strep supplementation. NK92 cells were a kind gift from Dr. Daniel Popkin (Innova Dermatology, TN, USA). They were cultured in Alpha-MEM media (Sigma-Aldrich) and supplemented with 12.5% FBS, 12.5% horse serum (Sigma-Aldrich), 0.02 mM folic acid (Acros), 0.1 mM 2-mercaptoethanol (Gibco), 1.5 g/L sodium bicarbonate (Fisher Scientific), 2 mM L-glutamine (Gibco), and 0.2 mM inositol (Acros). IL-2 (200 IU) was added fresh ever 2–3 days with media changes for NK92 cells. All cells were maintained at 37°C with 5% CO₂ in the cell incubator. Glucose concentrations of media used for cell culture: 2 g/L for primary NK cells, as they were in RPMI-1640 media; 1g/L for NK92 cells, as they were cultured in Alpha-MEM; and 4.5 g/L for HEK293T cells, as we use DMEM-High Glucose. All media are from Sigma-Aldrich as mentioned above.