Arpc1b

Manufactured by Merck Group

ARPC1B is a subunit of the Arp2/3 protein complex, which is involved in the regulation of actin polymerization and cytoskeletal organization. The Arp2/3 complex plays a crucial role in the formation of branched actin networks, which are essential for various cellular processes such as cell motility, endocytosis, and intracellular trafficking. ARPC1B is one of the seven subunits that make up the Arp2/3 complex and is responsible for connecting the complex to actin filaments.

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Lab products found in correlation

Ha tag, by Merck Group (1 mentions) Flag tag (flag), by Merck Group (1 mentions) Goat anti mouse igg h l secondary antibody, by Thermo Fisher Scientific (1 mentions) Goat anti rabbit igg h l secondary antibody, by Thermo Fisher Scientific (1 mentions) Tgn46, by Bio-Rad (1 mentions) β actin, by Proteintech (1 mentions) Calnexin, by Proteintech (1 mentions) Hsc70, by Santa Cruz Biotechnology (1 mentions) P16 arc, by Abcam (1 mentions) Myc tag, by Proteintech (1 mentions) Anti pan actin, by Cytoskeleton (1 mentions) α tublin, by Proteintech (1 mentions) His tag, by Proteintech (1 mentions) Rabex 5, by Santa Cruz Biotechnology (1 mentions) Capzβ, by Proteintech (1 mentions) Gapdh, by Proteintech (1 mentions) Flag m2, by Merck Group (1 mentions) Vinculin, by Merck Group (1 mentions) P erk1 2 t202 y204, by Cell Signaling Technology (1 mentions) P ndrg1 t346, by Cell Signaling Technology (1 mentions)

2 protocols using arpc1b

Comprehensive Protein Immunodetection in Cell Biology

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The primary antibodies were used as follows: GAPDH (Proteintech, 60004-1-Ig), CapZβ (Proteintech, 25043-1-AP), ARPC1B (Sigma-Aldrich, HPA004832), p16-Arc (Abcam, ab51243), N-WASP (Novus Biologicals, NBP1-82512), His-tag (ProteinTech, 66005-1-Ig), β-actin (ProteinTech, 60008-1-Ig), α-tublin (ProteinTech, 66031-1-Ig), VPS8 (ProteinTech, 15079-1-AP), λ-tublin (Sigma-Aldrich, T6557), HSC70 (Santa Cruz, sc-24), VPS34 (CST, 4263), CapZa1/a2 (Developmental Studies Hybridoma Bank, mAb 5B12.3), anti-pan actin (Cytoskeleton, AAN02-s), RAB5A (CST, 3547 or 46449), Calnexin (ProteinTech, 10427-2-AP), LAMP1 (CST, 9091), TGN46 (Bio-Rad, AHP500GT), EEA1 (CST, 3288), MYC-tag (ProteinTech, 67447-1-Ig), Flag-tag (Sigma-Aldrich, F3165), HA-tag (Sigma-Aldrich,11867423001), StrepMAB-Classic HRP conjugate (IBA, 2-1509-001), Rabex-5 (Santa Cruz, sc-166049), Rabaptin-5 (ProteinTech, 14350-1-AP), EGFR (Santa Cruz, sc-373746), goat anti-mouse IgG (H+L) secondary antibody (Invitrogen, 31430), goat anti-rabbit IgG (H+L) secondary antibody (Invitrogen, 31460), and goat anti-rat IgG (H+L) secondary antibody (Invitrogen, 31470).

Wang D., Ye Z., Wei W., Yu J., Huang L., Zhang H, & Yue J. (2021). Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors. eLife, 10, e65910.

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Western Blot Antibody Characterization

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Whole-cell lysates or immunoprecipitates were resolved by SDS-PAGE and blotted onto polyvinylidene difluoride (PVDF) membranes. Proteins were probed using primary antibodies obtained from the following sources (catalog number between brackets): pAKT (S473) (#9271), pAKT (T308) (#4056), AKT (#9272), pERK1/2 (T202/Y204) (#9106), p110α (#4249), PTEN (#9552), pFoxO1/3a (T24/T32) (#9464), pGSK3 (S21/9) (#9331), pS6 (S240/4) (#2215), pp70S6K (T389) (#9205), pPRAS40 (T246) (#2640), pNDRG1 (T346) (#3217), pAFAD (S1718) (#5485), pAAPK1 (S485) (#2537), AMPK (#2532) and GST (#2624) from Cell Signaling Technology; ARPC1B (#HPA004832), Vinculin (#V9131) and Flag M2 (#F3165) from Sigma; NT5C (#NBP1-84563) from Novus Biologicals; p34 (#07-227) from Millipore and AFAD (ab90809) from Abcam. A rabbit affinity-purified polyclonal antibody against NT5C pS184 (mouse NT5C amino acid numbering, equivalent to human NT5C pS182) was developed in collaboration with GenScript, utilizing the RLLpSWSDNWREILDC peptide, covering amino acids 179–192 of human NT5C. Quantification of western blots was performed using ImageJ.

Moniz L.S., Surinova S., Ghazaly E., Velasco L.G., Haider S., Rodríguez-Prados J.C., Berenjeno I.M., Chelala C, & Vanhaesebroeck B. (2017). Phosphoproteomic comparison of Pik3ca and Pten signalling identifies the nucleotidase NT5C as a novel AKT substrate. Scientific Reports, 7, 39985.

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