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Bm chemiluminescence substrate

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The BM Chemiluminescence Substrate is a laboratory reagent used in chemiluminescence-based assays. It is designed to produce a luminescent signal that can be detected and quantified, enabling the measurement of target analytes in a sample.

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Lab products found in correlation

Poly hrp40 streptavidin, by Biosynth (2 mentions) Infinite m200 pro plate reader, by Tecan (1 mentions) Fluostar microplate reader, by BMG Labtech (1 mentions)

Close spelling variants of this product

BM Chemiluminescence Blotting Substrate (POD) (16 citations) BM chemiluminescence blotting substrate (15 citations) BM Chemiluminescence Western Blotting Substrate (POD) (11 citations) BM Chemiluminescence ELISA substrate (5 citations) BM chemiluminescence western blotting substrate (4 citations) BM Chemiluminescence Blotting Substrate Kit (3 citations) BM Chemiluminescence Blotting Substrate (POD) Kit (3 citations) Chemiluminescence substrate (2 citations) BM Chemiluminescence ELISA Substrate (POD) (2 citations)

4 protocols using bm chemiluminescence substrate

1

Sensitive IgG ELISA for Transcytosis

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Example 9

Sensitive IgG ELISA after Transcytosis Assay

The entire procedure was performed at RT using an automated washer for the wash steps. A 384-well plate was coated with 30 μL/well of 1 μg/mL anti-human/mouse-IgG, Fcγ-specific in PBS for 2 hours followed by 1 hour incubation in blocking buffer PBS containing 1% BSA or 1 CroteinC for human and mouse IgG assays, respectively). Serially diluted samples from the transcytosis assay and standard concentrations of the antibody used in the transcytosis assay were added to the plate and incubated for 2 hours. After four washes, 30 μL/well of 50 ng/mL anti-human/mouse-F(ab)2-Biotin in blocking buffer was added and incubated for a further 2 hours. Following 6 washes, 30 μL/well of 50 ng/mL (huIgG assay) or 100 ng/mL (mIgG assay) Poly-HRP40-Streptavidin (Fitzgerald; in PBS containing 1% BSA and 0.05% Tween-20) was added and incubated for 30 min. After 4 washes, immune complexes were detected by addition of 30 μL/well of BM Chemiluminescence Substrate (Roche). The luminescence signal was measured using a luminescence plate reader and concentration calculated using the fitted standard curve. The sensitivity of the assay ranged from 10 pg/mL to 10 ng/mL.

US11584793B2. Anti-transferrin receptor antibodies with tailored affinity (2023-02-21). Hoffmann-La Roche Inc. [US]. Inventors: Stefan Dengl [DE], Guy Georges [DE], Ulrich Goepfert [DE], Jens Niewoehner [DE], Tilman Schlothauer [DE].
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2

Luminescence-based ELISA for Phl p 5-specific IgG1 and IgG2c

Cited 1 time
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Levels of Phl p 5-specific IgG1 and IgG2c were determined using a luminescence-based ELISA assay as previously described (Weinberger et al., 2013 (link)). In short, 96-well plates for immunoassays (Greiner) were coated for 24 h at 4°C with recombinant Phl p 5 (per well 50 μl of 1 μg/ml Phl p 5 in PBS). Afterwards, plates were washed with 0.1% Tween-20 in PBS (v/v) and incubated with blocking buffer (0.1% (v/v) Tween 20 and 2% (w/v) skim milk in PBS, pH 7.5) for 1 h at RT, before washing the plates again. Then, the plates were incubated with serum diluted (1:10,000) in blocking buffer for 1 h at RT, washed again, before the horse radish peroxidase (HRP)-conjugated antibodies for the detection of IgG1 (Zymed) or IgG2c (Zymed; diluted 1:1000 in blocking buffer) were added to the wells for 1 h at RT. After that, the luminometric assay (BM chemiluminescence substrate, Roche) was developed by adding the substrate (luminol diluted 1:2 in H2O) to each well. After 3 min incubation, chemiluminescence (photon counts/s) was determined using an Infinite M200 Pro Plate Reader (Tecan).
Klein B., Mrowetz H., Thalhamer J., Scheiblhofer S., Weiss R, & Aigner L. (2016). Allergy Enhances Neurogenesis and Modulates Microglial Activation in the Hippocampus. Frontiers in Cellular Neuroscience, 10, 169.
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3

Quantifying Influenza-Specific Antibodies by ELISA

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HA and NA specific immunoglobulin G (IgG) titres were determined by ELISA. Briefly, Nunc TM MaxiSorp TM plates were coated with active NA [A/Anhui/1/2005 (H5N1) or A/California/04/2009 (H1N1) pdm09, Sino Biological Inc., USA] or HA [A/Viet Nam/1203/2004 (H5N1), Sino Biological Inc., USA]. In consecutive order, with wash steps in between, plates were incubated with blocking buffer, 5fold serial dilutions of ferret sera, horse radish peroxidase conjugated goat-anti-ferret IgG antibody (Novus Biologicals, Littleton, USA), and either BM Chemiluminescence substrate (Roche, Germany) or 3,3 0 ,5,5 0 -Tetramethylbenzidine (TMB) One Substrate (KemEnTech, Denmark). Chemiluminescence was measured immediately, whereas the TMB reaction was stopped with H 2 SO 4 and absorbance read at 450 nm using 620 nm as a reference. Both substrate read-outs were measured on a FLUOstar Microplate Reader (BMG LABTECH, Germany).
Guilfoyle K., Major D., Skeldon S., James H., Tingstedt J.L., Polacek C., Lassauniére R., Engelhardt O.G, & Fomsgaard A. (2021). Protective efficacy of a polyvalent influenza A DNA vaccine against both homologous (H1N1pdm09) and heterologous (H5N1) challenge in the ferret model. Vaccine, 39(34).
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4

Sensitive IgG ELISA for Transcytosis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 9

Sensitive IgG ELISA after Transcytosis Assay

The entire procedure was performed at RT using an automated washer for the wash steps. A 384-well plate was coated with 30 μL/well of 1 μg/mL anti-human/mouse-IgG, Fcγ-specific in PBS for 2 hours followed by 1 hour incubation in blocking buffer PBS containing 1% BSA or 1 CroteinC for human and mouse IgG assays, respectively). Serially diluted samples from the transcytosis assay and standard concentrations of the antibody used in the transcytosis assay were added to the plate and incubated for 2 hours. After four washes, 30 μL/well of 50 ng/mL anti-human/mouse-F(ab)2-Biotin in blocking buffer was added and incubated for a further 2 hours. Following 6 washes, 30 μL/well of 50 ng/mL (huIgG assay) or 100 ng/mL (mIgG assay) Poly-HRP40-Streptavidin (Fitzgerald; in PBS containing 1% BSA and 0.05% Tween-20) was added and incubated for 30 min. After 4 washes, immune complexes were detected by addition of 30 μL/well of BM Chemiluminescence Substrate (Roche). The luminescence signal was measured using a luminescence plate reader and concentration calculated using the fitted standard curve. The sensitivity of the assay ranged from 10 pg/mL to 10 ng/mL.

US10323089B2. Anti-transferrin receptor antibodies with tailored affinity (2019-06-18). Hoffmann-La Roche Inc. [US]. Inventors: Stefan Dengl [DE], Guy Georges [DE], Ulrich Goepfert [DE], Jens Niewoehner [DE], Tilman Schlothauer [DE].
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