N succinimidyl dota nhs dota

Manufactured by Macrocyclics

Sourced in United States

N-succinimidyl-DOTA (NHS-DOTA) is a bifunctional chelating agent used for the conjugation of proteins, peptides, or other biomolecules to metal ions. It contains a succinimidyl ester group that can form a covalent bond with primary amino groups, and a DOTA (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid) group that can chelate metal ions.

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Lab products found in correlation

Keytruda, by Merck Group (1 mentions) Oxalic acid, by PerkinElmer (1 mentions)

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DOTA-NHS (22 citations)

2 protocols using n succinimidyl dota nhs dota

PD-1 Antibody Radiolabeling Protocol

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Pembrolizumab (25 mg/mL, Keytruda®; Merck & Co., Kenilworth, NJ), a humanized monoclonal immunoglobulin IgG4 antibody directed against human PD-1 (hPD-1), was purchased from Stanford University Hospital Pharmacy (Stanford, CA). The radioisotope chelator N-succinimidyl-DOTA (NHS-DOTA) was purchased from Macrocyclics (Dallas, TX). ⁶⁴Cu CuCl₂ was purchased from the University of Wisconsin (Madison, WI). All other experimental reagents were obtained from Sigma-Aldrich (St. Louis, MO) unless otherwise stated.

Natarajan A., Patel C.B., Habte F, & Gambhir S.S. (2018). Dosimetry Prediction for Clinical Translation of 64Cu-Pembrolizumab ImmunoPET Targeting Human PD-1 Expression. Scientific Reports, 8, 633.

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Radiolabeling of Pembrolizumab with Zirconium-89 and Copper-64

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All reagents were obtained from Sigma-Aldrich (St. Louis, MO) unless otherwise stated. The chelators P-isothiocyanatobenzyl-desferrioxamine (Df-Bz-NCS: Df) and N-succinimidyl-DOTA (NHS-DOTA) were purchased from Macrocyclics (Dallas, TX, USA). [⁸⁹Zr] in 1 M Oxalic acid (t _½ = 78.4 h, radionuclide purity >99%; 1-0.8 GBq/ml in 1 M Oxalic acid) was obtained from Perkin Elmer (Waltham, MA, USA). [⁶⁴Cu]Cl₂ was purchased from University of Wisconsin (Madison, WI). A humanized monoclonal immunoglobulin (Ig) G4 antibody (25 mg/ml) directed against human PD-1 (keytruda^®[Pembrolizumab], Merck & Co., Rahway, NJ, USA) was purchased from Stanford University Hospital Pharmacy (Stanford, CA, USA).

Natarajan A., Mayer A.T., Reeves R.E., Nagamine C.M, & Gambhir S.S. (2017). Development of a novel immunoPET tracer to image human PD-1 checkpoint expression on tumor infiltrating lymphocytes in a humanized mouse model. Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging, 19(6), 903-914.

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