Protein samples were extracted from graft tissues. Equal masses of protein samples were separated by SDS-PAGE, and transferred to PVDF membranes that were blocked and reacted with primary antibodies according to the manufacturer's recommendations. The antibodies used include: CDKN2A (1:600, Abcam), TNFAIP6 (1:800, Abcam), ESR2 (1:400, Abcam), UCN (1:400, LifeSpan BioSciences), IL1B (1:1000, Abcam), VIP (1:1000, Abcam), NFKBIA (1:1000, Abcam), GAL (1:600, Santa), CSF1 (1:400, Santa), JUN (1:1000, Abcam), ARG1 (1:800, Abcam), FGF2 (1:1000, Abcam), ATF3 (1:1000, Abcam), ZFP36 (1:400, Millipore), CCL2 (1:1000, Millipore), GFI1 (1:400, Abcam), CCK (1:1000, Santa), RUNX2 (1:600, Abcam), TNFα (1:600, Abcam), and β-actin (1:4,000, Proteintech). The specific binding of primary antibody was detected by HRP-conjugated species-specific secondary antibody (Beyotime, Shanghai, China) and enhanced chemiluminescence (ECL) assay.
Hrp conjugated species specific secondary antibody
Manufactured by Beyotime
Sourced in China
HRP-conjugated species-specific secondary antibody is a laboratory reagent used in various immunoassays, such as Western blotting and ELISA. It consists of a secondary antibody that is conjugated with the enzyme Horseradish Peroxidase (HRP). This product can be used to detect and quantify target proteins or other biomolecules in a sample by binding to a primary antibody that is specific to the molecule of interest.
Automatically generated - may contain errors
Lab products found in correlation
Gapdh, by Proteintech (2 mentions)
Runx2, by Abcam (1 mentions)
Cdkn2a, by Abcam (1 mentions)
Tnf α, by Abcam (1 mentions)
Il 1b, by Abcam (1 mentions)
Fibroblast growth factor 2 (fgf2), by Abcam (1 mentions)
Arg 1, by Abcam (1 mentions)
β actin, by Proteintech (1 mentions)
Rab8a, by Abcam (1 mentions)
Rab11a, by Abcam (1 mentions)
Cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Bcl 2, by Proteintech (1 mentions)
3 protocols using hrp conjugated species specific secondary antibody
1
Protein Expression Analysis in Graft Tissues
2
Immunoblotting of Rab GTPases
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Protein samples were extracted from cell cultures. Equal amount of protein samples were separated by SDS-PAGE, and transferred to PVDF membranes, which were blocked, and reacted with primary antibodies against Rab8a (1:1000, Abcam, Cambridge, UK), Rab11a (1 : 500, Abcam), or GAPDH (1:1000, Proteintech, Wuhan, China) according to the manufacturer's recommendations. The specific binding of primary antibody was detected by HRP-conjugated species-specific secondary antibody (Beyotime, Shanghai, China) and enhanced chemiluminescence assay.
3
Western Blot Analysis of Apoptosis Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Extracted from cell cultures, the protein samples were separated by SDS-PAGE and transferred to the PVDF membranes. According to the manufacturer’s recommendations, the PVDF membranes were then blocked and incubated with the primary antibodies against PAX3 (1:500, Abcam), p53 (1:200, Cell Signaling Technology), Cleaved Caspase-3 (1:500, Cell Signaling Technology), Bcl-2 (1:500, Proteintech) or GAPDH (1:1000, Proteintech). Via HRP-conjugated species-specific secondary antibody (Beyotime, Shanghai, China) and enhanced chemiluminescence assay, the specific binding of primary antibody was finally detected (Zhang et al., 2016 (link)).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!