Cells at logarithmic growth stage were taken and seeded in 96-well plates with 4 × 103–1×105 cells per well. The cells were cultured to the normal growth stage. Transfection was performed according to experimental requirements. An appropriate amount of 50 μM Edu culture medium (Ribobio, Guangzhou) was prepared by diluting Edu solution 1,000:1. The cells were incubated at 20 μM Edu for 12 h. The cells were then fixed with 4% paraformaldehyde, stained with DAPI for 5 min, and cell proliferation was recorded by taking photos with a microscope.
Edu culture medium
Manufactured by RiboBio
Edu culture medium is a laboratory cell culture solution designed for the cultivation of various cell types. It provides the necessary nutrients and growth factors to support cell growth and proliferation in an in vitro environment.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using edu culture medium
1
Cell Proliferation Quantification via EdU Labeling
2
Quantifying Cell Proliferation by EdU Incorporation
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Cells at logarithmic growth stage were taken and seeded in 96-well plates with 4×103~1×105 cells per well. The cells were cultured to the normal growth stage. Transfection was performed according to experimental requirements. An appropriate amount of 50 μM Edu culture medium (Ribobio, Guangzhou) was prepared by diluting Edu solution 1000:1. The cells were incubated at 20 μM Edu for 12 h. The cells were then fixed with 4% paraformaldehyde, stained with DAPI for 5 minutes, and cell proliferation was recorded by taking photos with a microscope.
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