Pe cy5 cd86

Manufactured by BioLegend

Sourced in United States

PE/Cy5-CD86 is a fluorescent-conjugated antibody that binds to the CD86 cell surface protein. CD86 is a co-stimulatory molecule expressed on antigen-presenting cells and plays a role in T cell activation. The PE/Cy5 fluorescent label allows for detection and analysis of CD86-expressing cells using flow cytometry.

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CD86-PE (65 citations) PE-Cy5-conjugated anti-CD86 Ab (3 citations)

3 protocols using pe cy5 cd86

Characterizing B Cell Subsets in SLE

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To detect subpopulations and characterize phenotypes of B cells, PBMCs from SLE patients (n = 20) were separated and analyzed to determine the frequency of each B cell subset. Two × 10⁵ cells were resuspended in 50 μl FACS buffer and incubated with surface antibodies for 15 min at 4 °C. Antibodies used included FITC-CD19, APC-CD21, APC/Fire750-CD27, Alexa/fluorro700-CD11c, PerCP/Cy5.5-CXCR5, PerCP/Cy5.5-IgG, PE/Cy7-IgD, APC-HLA-DR, PE/Cy5-CD69, and PE/Cy5-CD86 (Biolegend, San Diego, USA). After staining, cells were washed with 1 ml FACS buffer and resuspended in 300 μl FACS buffer for surface marker analyses.
The frequency and phenotype of DNA tetramer-binding B cells were detected by the tetramer staining technique, resuspended in 100 μl FACS buffer, and incubated with surface antibodies for 15 min at 4 °C. The stained B cells were washed with 1 ml FACS buffer and resuspended in 500 μl FACS buffer for flow cytometric surface marker analysis. FACS data were acquired with a BD FACS Canto II (Becton-Dickinson Immunocytometry Systems, San Jose, USA) and analyzed with FlowJo software (v. 10.0; Tree Star Inc., CA, USA).

Wangriatisak K., Thanadetsuntorn C., Krittayapoositpot T., Leepiyasakulchai C., Suangtamai T., Ngamjanyaporn P., Khowawisetsut L., Khaenam P., Setthaudom C., Pisitkun P, & Chootong P. (2021). The expansion of activated naive DNA autoreactive B cells and its association with disease activity in systemic lupus erythematosus patients. Arthritis Research & Therapy, 23, 179.

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Multifunctional flow cytometry for EGFR-specific CTL

Cited 2 times

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Recombinant epidermal growth factor, Zombie Aqua^™ Fixable Viability Kit, Alexa Fluor 488-EGFR, PerCP-Cy 5.5-CD3, PerCP-Cy 5.5-CD32 and PE-Cy5-CD86 flow antibodies were purchased from Biolegend (San Diego, CA). Cetuximab and panitumumab were purchased from the manufacturers, Bristol-Myers Squibb (New York, NY), and Amgen, (Thousand Oaks, California) respectively. FITC-CD69 and PE-Texas Red-CD8 flow antibody were purchased from Life Technologies (Grand Island, NY). PE-CD107a, PE-Cy5-CD137, PE-Cy7-CD16, APC-CD56, PE-CD32, APC-H7-HLA-A2 and FITC-CD80 flow antibodies were purchased from BD Biosciences (San Jose, CA). 12B6 antibody was produced by Dr. Ferrone (Harvard Medical School) and has been previously validated (14 (link)). PE-labeled HLA-A*0201-EGFR853-861 tetramer was provided by the NIH tetramer core facility and used for identification of EGFR-specific CTL (15 (link)). PE-Labeled HLA-A*02:01 HIV-tetramer was purchased from MBL International.

Trivedi S., Srivastava R.M., Concha-Benavente F., Ferrone S., Garcia-Bates T.M., Li J, & Ferris R.L. (2016). Anti-EGFR targeted monoclonal antibody isotype influences anti-tumor cellular immunity in head and neck cancer patients. Clinical cancer research : an official journal of the American Association for Cancer Research, 22(21), 5229-5237.

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Phenotypic Profiling of B Cells in SLE

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To detect subpopulations and characterize phenotypes of B cells, PBMCs from SLE patients (n = 20) were separated and analyzed to determine the frequency of each B cell subset. Two x 10 5 cells were resuspended in 50 µl FACS buffer and incubated with surface antibodies for 15 min at 4 o C. Antibodies used included FITC-CD19, APC-CD21, APC/Fire750-CD27, Alexa/ uorro700-CD11c, PerCP/Cy5.5-CXCR5, PerCP/Cy5.5-IgG, PE/Cy7-IgD, APC-HLA-DR, PE/Cy5-CD69, and PE/Cy5-CD86 (Biolegend, San Diego, USA). After staining, cells were washed with 1 ml FACS buffer and resuspended in 300 µl FACS buffer for surface marker analyses.
The frequency and phenotype of DNA tetramer-binding B cells were detected by the tetramer staining technique, resuspended in 100 µl FACS buffer, and incubated with surface antibodies for 15 min at 4 o C.
The stained B cells were washed with 1 ml FACS buffer and resuspended in 500 µl FACS buffer for ow cytometric surface marker analysis. FACS data were acquired with a BD FACS Canto II (Becton-Dickinson Immunocytometry Systems, San Jose, USA) and analyzed with FlowJo software (v. 10.0; Tree Star Inc., CA, USA).

Wangriatisak K., Thanadetsuntorn C., Krittayapoositpot T., Leepiyasakulchai C., Suangtamai T., Ngamjanyaporn P., Khowawisetsut L., Khaenam P., Setthaudom C., Pisitkun P., & Chootong P. (2021). The expansion of activated naive DNA autoreactive B Cells and its association with disease activity in Systemic Lupus Erythematosus patients.

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