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Las 4.4 imaging system

Manufactured by Leica

The Leica LAS 4.4 imaging system is a comprehensive software suite designed for advanced microscopy and image analysis. It provides a robust platform for acquiring, processing, and analyzing high-quality digital images from a variety of Leica microscope systems. The LAS 4.4 software offers an intuitive user interface and a wide range of tools for image capture, enhancement, and quantitative analysis, supporting researchers and scientists in various fields of study.

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2 protocols using las 4.4 imaging system

1

Specimen Imaging and Preservation

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Specimens were point mounted on black, acid-free card for examination (using a Leica M205C stereomicroscope with LED light source), photography and long term preservation. Images were acquired using the Leica LAS 4.4 imaging system, which comprised a Leica® Z16 microscope with a Leica DFC450 Camera with 0.63× video objective attached. The imaging process, using an automated Z-stepper, was managed using the Leica Application Suite V 4.4 software installed on a desktop computer. Lighting was achieved using techniques summarized in Buffington et al. (2005) , Kerr et al. (2008) and Buffington and Gates (2009) . All images presented in this paper are available at http://www.waspweb.org.
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2

Microscopy Imaging Protocol for Wasps

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At SAMC we used a Leica LAS 4.4 imaging system, which comprised a Leica® Z16 microscope with a Leica DFC450 Camera with 0.63× video objective attached. The PageBreakimaging process, using an automated Z-stepper, was managed using the Leica Application Suite V 4.4 software installed on a desktop computer. At BMNH, images were acquired using a Canon SLR EOS 5DSR with 65 mm macro lens mounted on a copy stand with an automated Z-stepper; images were aligned using Helicon Focus software version 6.6.1. Diffused lighting was achieved using techniques summarized in Buffington et al. (2005) , Kerr et al. (2008) , and Buffington and Gates (2009) . All images presented in this paper, as well as supplementary images, are available at www.waspweb.org.
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