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2 protocols using horseradish peroxidase hrp linked horse anti mouse igg

1

Characterization of GPR64 Receptor

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Reagents and antibodies were purchased from the following companies: forskolin (Sigma, St. Louis, MO, USA; #F6886), probenecid (Sigma; #P8761), calcium chloride (Sigma; #21115), H-89 (Cell Signaling Technologies, Beverly, MA, USA; #9844), U0126 (Cell Signaling Technologies; #9903), collagenase (Sigma; #C2674), zeocin (Thermo Fisher Scientific, Waltham, MA, USA; #R25001), Poly-D-Lysine (Sigma; #6407), mouse anti-FLAG (Cell Signaling Technologies; #8146), rabbit anti-HA (Cell Signaling Technologies; #3724), mouse anti-V5 (Thermo Fisher Scientific; #R960–25), rabbit anti-N-terminal epitope of GPR64 (Atlas Antibodies AB, Bromma, Sweden; #HPA001478), mouse anti-CaSR antibody (Thermo Fisher Scientific; #MA1–934), rabbit anti-β-actin (Sigma; #A2066), horseradish peroxidase (HRP)-linked horse anti-mouse IgG (Cell Signaling Technologies; #7076), and HRP-linked goat anti-rabbit IgG (Cell Signaling Technologies; #7074).
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2

Antibody Detection Protocols for DNase I and TRAP1

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The following primary antibodies were used in this study: rabbit anti‐DNase I (LS‐B4846; LifeSpan BioSciences, Seattle, WA, USA, sc‐30058; Santa Cruz Biotechnology, Heidelberg, Germany, ab113241; Abcam), mouse anti‐DNase I (sc‐376207; Santa Cruz Biotechnology), and goat anti‐TRAP1 (sc69289; Santa Cruz Biotechnology). Horseradish peroxidase (HRP)‐linked horse anti‐mouse IgG (7076; Cell Signaling Technology, Leiden, The Netherlands), HRP‐conjugated goat anti‐rabbit IgG (65‐6120; Invitrogen, Carlsbad, CA, USA), Alexa 594‐conjugated chicken anti‐rabbit IgG (A‐21442), Alexa 488 F(ab′)2 ‐conjugated goat anti‐rabbit IgG (A‐11070), and Alexa 488‐conjugated donkey anti‐goat IgG (A‐11055) were used as secondary antibodies (all three from Thermo Fisher Scientific, Waltham, MA, USA).
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