Wool was purchased from sheep farmers from Romania. Chemical reagents of analytical grade like sodium hydroxide, ammonia (25%), sodium carbonate, formic acid, and sulphuric acid were purchased from Chemopar SA (Bucharest, Romania). Borron SE (ethoxylated alkyl derivatives with 65% concentration) was supplied by SC Triderma SRL (Bucharest, Romania). Promatex®, an endo-protease from Bacillus spp with the activity of 1.5 AU-A/g, in the range of pH = 6–9 and temperature of 30–65 °C and Esperase® 8.0 L, a serine endo-peptidase from Bacillus lentus with the activity of 8 KNPU-E/g, working at elevated temperature and pH = 8–12.5, were purchased from Novozymes (Atasehir, Turkey). Valkerase®, a keratinase, serine protease from B. licheniformis, with activity of 80,549 U/g at pH = 5.5 and 55 °C, was supplied by BioResource International (Durham, NC, USA).
Maize seeds (Zea mays L.) were supplied by Probstdorfer Saatzucht Romania SRL (Bucharest, Romania), namely the Olt hybrid, FAO 430, the same one used in the field, in the micro- and macroplots.
Maize seeds (Zea mays L.) were supplied by Probstdorfer Saatzucht Romania SRL (Bucharest, Romania), namely the Olt hybrid, FAO 430, the same one used in the field, in the micro- and macroplots.