Mpdca1

Manufactured by BioLegend

The MPDCA1 is a lab equipment product. It is a multi-parameter digital cell analyzer used for the detection and analysis of cells.

Automatically generated - may contain errors

Lab products found in correlation

Mhcii 1 a 1 e, by BioLegend (2 mentions) Live dead fixable near ir dead cell staining kit, by Thermo Fisher Scientific (2 mentions) Siglec h, by BioLegend (2 mentions) Anti mouse cd11c, by BioLegend (2 mentions) Pe conjugated anti mouse ly49q, by MBL Life Science (2 mentions) Cd11b, by BioLegend (2 mentions) Facscanto 2, by BD (1 mentions) Magnetic activated cell sorting (macs), by Miltenyi Biotec (1 mentions) Facsaria, by BD (1 mentions)

3 protocols using mpdca1

Fluorescent Antibody-Based Immune Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fluorescent-conjugated antibodies including anti-mouse CD11c, CD11b,
B220, SiglecH, mPDCA1, and MHCII (I-A/I-E) were purchased from Biolegend (San
Diego, CA). PE-conjugated anti-mouse Ly49Q was purchased from MBL International
(Woburn, MA). LIVE/DEAD fixable near-IR dead cell staining kit was purchased
from Life Technologies (Grand Island, NY).

Scott J.L., Wirth J.R., EuDaly J.G., Gilkeson G.S, & Cunningham M.A. (2016). Plasmacytoid dendritic cell distribution and maturation are altered in lupus prone mice prior to the onset of clinical disease. Clinical immunology (Orlando, Fla.), 175, 109-114.

+ Open protocol

+ Expand

Comprehensive Immune Cell Phenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fluorescent conjugated antibodies including anti-mouse CD11c, CD11b, B220, SiglecH, mPDCA1, PDC-TREM, MHC II (I-A/I-E), and CD40 were purchased from Biolegend (San Diego, CA). PE-conjugated anti-mouse Ly49Q was purchased from MBL International (Woburn, MA). LIVE/DEAD fixable near-IR dead cell staining kit was purchased from Life Technologies (Grand Island, NY). Mouse CpG DNA was purchased from Hycult Biotech (Canton, MA).

Scott J.L., Cunningham M.A., Naga O.S., Wirth J.R., EuDaly J.G, & Gilkeson G.S. (2015). Estrogen receptor alpha deficiency modulates TLR ligand mediated PDC-TREM expression in plasmacytoid dendritic cells in lupus prone mice. Journal of immunology (Baltimore, Md. : 1950), 195(12), 5561-5571.

+ Open protocol

+ Expand

Multiparametric Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were analysed on a FACSCanto II (BD). DAPI was added for dead cell discrimination. FACS sorting was performed on a FACSAria (BD) after MACS (Miltenyi Biotec) depletion of CD3ε/CD19⁺ cells. Monoclonal antibodies against B220, CD3ε, CD11b, CD11c, CD19, CD27, CD86, and CD16/32 were purchased from BD, mPDCA-1 was purchased from BioLegend.

Ali S., Hoven A., Dress R.J., Schaal H., Alferink J, & Scheu S. (2018). Identification of a novel Dlg2 isoform differentially expressed in IFNβ-producing plasmacytoid dendritic cells. BMC Genomics, 19, 194.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!