Anti mouse igg minus

Manufactured by Merck Group

The Anti-mouse IgG MINUS is a laboratory reagent used to detect and quantify mouse immunoglobulin G (IgG) in samples. It functions as a secondary antibody that binds to mouse IgG, enabling its identification and measurement in various immunoassay techniques.

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Lab products found in correlation

Anti rabbit igg plus pla probes, by Merck Group (2 mentions) Duolink in situ detection reagents red, by Merck Group (2 mentions)

Spelling variants of this product

Anti-mouse MINUS (22 citations) Anti-IgG mouse (5 citations) IgG mouse (3 citations)

2 protocols using anti mouse igg minus

Duolink PLA for Protein Interactions

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For the Duolink in situ proximity ligation assay (PLA), sections were incubated for 90 min with oligonucleotide-conjugated anti-mouse IgG MINUS (DUO92004; Sigma; MO) and anti-rabbit IgG PLUS (PLA probes; DUO92002; Sigma; MO) diluted 1:6 in Tris-buffered saline at 37°C. Amplified DNA strands were detected with oligonucleotides conjugated to a fluorophore (Duolink In Situ Detection Reagents Red, DUO92008, Sigma; MO). They were cover-slipped with mounting medium DAPI (DUO82040; Sigma; MO) and analyzed by confocal microscopy.

Verma N., Ly H., Liu M., Chen J., Zhu H., Chow M., Hersh L.B, & Despa F. (2016). Intraneuronal Amylin Deposition Peroxidative Membrane Injury and Increased IL-1β Synthesis in Brains of Alzheimer’s Disease Patients with Type-2 Diabetes and in Diabetic HIP Rats. Journal of Alzheimer's disease : JAD, 53(1), 259-272.

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Proximity Ligation Assay for Protein Interactions

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For the Duolink in situ proximity ligation assay (PLA), sections were incubated for 90 min with oligonucleotide-conjugated anti-mouse IgG MINUS (DUO92004; Sigma-Aldrich, St. Louis, MO) and anti-rabbit IgG PLUS (PLA probes; catalog #DUO92002, Sigma-Aldrich) diluted 1:6 in Tris-buffered saline at 37°C. Amplified DNA strands were detected with oligonucleotides conjugated to a fluorophore (Duolink In Situ Detection Reagents Red; catalog #DUO92008; Sigma-Aldrich). They were coverslipped with mounting medium DAPI (catalog #DUO82040; Sigma-Aldrich) and analyzed by confocal microscopy.

Liu M., Verma N., Peng X., Srodulski S., Morris A., Chow M., Hersh L.B., Chen J., Zhu H., Netea M.G., Margulies K.B., Despa S, & Despa F. (2016). Hyperamylinemia Increases IL-1β Synthesis in the Heart via Peroxidative Sarcolemmal Injury. Diabetes, 65(9), 2772-2783.

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