TAK901 (#T2709) was purchased from TargetMol (Shanghai, China). Primary antibodies were as follows: SREBP-1 (2A4) (#sc-13551, Santa Cruz Biotechnology, Santa Cruz, CA, USA), SREBP-2 (1C6) (#sc-13552, Santa Cruz Biotechnology, Santa Cruz, CA, USA), phospho-GSK-3β (Ser9) (D85E12) (#5558, CST, Danvers, MA, USA), β-actin (#20536-1-AP, Proteintech, Wuhan, China), Ki67 (Servicebio, Wuhan, China),histone H3 (#4499s, CST, Danvers, MA, USA), phospho-histone H3 (Ser10) (#53348T, CST, Danvers, MA, USA), Aurora A (#45-8900, Invitrogen, Carlsbad, CA, USA), Aurora B (#ab2254, abcam, Waltham, MA, USA) and c-Myc (#9402S, CST, Danvers, MA, USA). Secondary antibodies were as follows: anti-mouse IgG, HRP-linked antibody (#7076, CST, Danvers, MA, USA), anti-rabbit IgG, HRP-linked antibody (#7074, CST, Danvers, MA, USA).
Srebp 2 1c6
Manufactured by Santa Cruz Biotechnology
Sourced in United States, United Kingdom
SREBP-2 (1C6) is a mouse monoclonal antibody that recognizes the sterol regulatory element-binding protein 2 (SREBP-2). SREBP-2 is a transcription factor that plays a key role in the regulation of cholesterol homeostasis by controlling the expression of genes involved in cholesterol biosynthesis and uptake.
Automatically generated - may contain errors
Lab products found in correlation
Aurora b, by Abcam (1 mentions)
Srebp 1 2a4, by Santa Cruz Biotechnology (1 mentions)
Aurora a, by Thermo Fisher Scientific (1 mentions)
β actin, by Proteintech (1 mentions)
Act 2d7, by Euromedex (1 mentions)
Enhanced chemiluminescence, by GE Healthcare (1 mentions)
Apolipoprotein b, by Abcam (1 mentions)
Anti srebp1 2a4, by Santa Cruz Biotechnology (1 mentions)
3 protocols using srebp 2 1c6
1
Molecular Signaling Pathways in Cancer
2
SDS-PAGE and Western Blot Analysis of STARD3, VAP-A/B, and SREBP-2
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SDS–PAGE and Western blot analysis were performed as previously described (Alpy et al, 2005 ) using the following antibodies: STARD3: pAbMLN64‐Ct‐605 (Moog‐Lutz et al, 1997 ); pAbMLN64‐Nt‐1611 (Alpy et al, 2001 ); VAP‐A: K‐15 sc‐48698 (Santa Cruz biotechnology); VAP‐B: rabbit anti‐VAP‐B (Kabashi et al, 2013 ); SREBP‐2 1C6 (sc‐13552; Santa Cruz biotechnology); actin: ACT‐2D7 (Euromedex).
3
Immunoblotting of SREBP Proteins
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Equal amounts of protein were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS/PAGE), transferred to Immobilon PVDF membranes, and probed with anti-SREBP-1 (2A4) (Santa Cruz Biotechnology, Santa Cruz, CA, USA), apolipoprotein B (Abcam, Cambridge, UK), and SREBP-2 (1C6) (Santa Cruz Biotechnology) antibodies. The secondary antibodies used were anti-mIgGj BP antibody (Santa Cruz Biotechnology) and goat anti-rabbit antibody (Jackson ImmunoResearch, West Grove, PA, USA) conjugated to horseradish peroxidase. Immunoreactive bands were visualized by enhanced chemiluminescence (GE Healthcare, Waukesha, WI, USA).
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