3-d-old dark-grown seedlings were bisected longitudinally and incubated in 2 M KOH at room temperature for 1 h and then in 1% Tween 20 for 30 min. After washing with double-distilled H2O (ddH2O) until pH 7.0 was reached, slices were placed between glass slides, and a load of 5 g was applied for 5 min. The innermost wall layer of primary cell walls was examined by scanning probe atomic force microscope (Bruker MultiMode V with NanoScope V Controller and SCANSYST-AIR probe). Contact AFM was performed in air at room temperature. Images of 2 µm2 size with 512 × 512-pixel resolution were recorded using the software NanoScope Analysis (Version 1.10). At least five areas per cell were scanned, and at least six cells from three samples were analyzed.
Scansyst air probe
Manufactured by Bruker
Sourced in China
The SCANSYST-AIR probe is a non-contact surface analysis tool designed for the characterization of surfaces. It measures the topography and surface properties of samples without physically contacting the surface, allowing for non-destructive analysis.
Automatically generated - may contain errors
2 protocols using scansyst air probe
1
Visualization of Primary Cell Wall Structure
2
Fluorescence and AFM Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All the fluorescence measurements were performed on a Hitachi F-7000 spectrofluorimeter (Hitachi, Tokyo, Japan). The excitation wavelength was 418 nm, and the spectra were recorded between 570 and 660 nm. The fluorescence emission intensity was measured at 595 nm.
All the AFM measurements were conducted using a Multimode 8 AFM with a Nanoscope V Controller (Bruker Corporation, Beijing, China) in ScanAsyst mode. The measurements were performed in air using a SCANSYST-AIR probe supplied by Bruker Corporation, (Beijing, China).
All the AFM measurements were conducted using a Multimode 8 AFM with a Nanoscope V Controller (Bruker Corporation, Beijing, China) in ScanAsyst mode. The measurements were performed in air using a SCANSYST-AIR probe supplied by Bruker Corporation, (Beijing, China).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!