Anxa5 protein

Human gastric cancer cell lines MGC-803 and MKN-45 were obtained from Shanghai Academy of Medical Sciences (Shanghai, China). The cells were incubated at 37°C in 5% CO₂ in plastic tissue culture flasks (Corning Inc., Acton, MA, USA) with complete Dulbecco’s modified Eagle’s medium (DMEM) (Gibco-BRL, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS; Gibco-BRL). ANXA5, PCNA, Bcl-2, Bax, MMP-9, E-cadherin, β-actin, GAPDH, MEK1/2, pMEK1/2, ERK1/2, and pERK1/2 monoclonal antibody were purchased from Sigma (St. Louis, MO, USA) and the ANXA5 protein was bought from novoprotein (Shanghai, China); the Trizol reagent, protein assay kit, the siRNA targeted to ANXA5 and the negative control siRNA were purchased from Invitrogen Life Technologies (Carlsbad, CA, USA); the gel image analysis system and electrophoresis apparatus were purchased from Bio-Rad (Hercules, CA, USA). The digital image analysis system and the fluorescence microscope were from Nikon (Tokyo, Japan), and the ultraviolet analyzer was from Beckman Coulter (Miami, FL, USA).

Human gastric cancer cell lines MGC-803 and MKN-45 were obtained from Shanghai Academy of Medical Sciences (Shanghai, China). The cells were incubated at 37°C in 5% CO₂ in plastic tissue culture flasks (Corning Inc., Acton, MA, USA) with complete Dulbecco’s modified Eagle’s medium (DMEM) (Gibco-BRL, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS; Gibco-BRL). ANXA5, PCNA, Bcl-2, Bax, MMP-9, E-cadherin, β-actin, GAPDH, MEK1/2, pMEK1/2, ERK1/2, and pERK1/2 monoclonal antibody were purchased from Sigma (St. Louis, MO, USA) and the ANXA5 protein was bought from novoprotein (Shanghai, China); the Trizol reagent, protein assay kit, the siRNA targeted to ANXA5 and the negative control siRNA were purchased from Invitrogen Life Technologies (Carlsbad, CA, USA); the gel image analysis system and electrophoresis apparatus were purchased from Bio-Rad (Hercules, CA, USA). The digital image analysis system and the fluorescence microscope were from Nikon (Tokyo, Japan), and the ultraviolet analyzer was from Beckman Coulter (Miami, FL, USA).