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Baf s1413

Manufactured by Selleck Chemicals

The Baf (S1413) is a laboratory instrument designed for the analysis and separation of biological samples. It is a compact and versatile device that can be used for a variety of applications in research and clinical settings. The core function of the Baf (S1413) is to provide efficient and reliable separation of complex mixtures, allowing researchers to isolate and analyze specific components of interest. Its performance and capabilities are well-suited for various biochemical and analytical procedures.

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2 protocols using baf s1413

1

Comprehensive Antibodies and Reagents Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total β-catenin antibody (1:1,000) was purchased from Invitrogen (712700), glyceraldehyde-3-phosphate dehydrogenase antibody (1:1,000) and FAK antibody (1:1,000, 3285) were obtained from Cell Signaling Technologies, anti-ATP6V0a3 antibody (1:500) was obtained from Novus (nbp1–89333, 1:1,000). CD63 antibody was obtained from Abcam.
Antibodies against Pak1 (ab131522), Ras (ab52939), GSK3 (ab93926, 1:4000), and secondary antibodies for immunostaining for cells (ab150120, ab150084, ab150117, ab150081) (1:300) were obtained from Abcam. Antibody against the focal adhesion protein Tes (HPA018123, 1:100) was obtained from Atlas antibodies. Secondary antibodies for immunostaining arrays (A-11001, A-11011 were obtained from Invitrogen). HRP-linked secondary antibodies (7076, 7074 at 1:5000 (Cell Signaling) were used for Western blots and analyzed with an iBright Imaging system. EIPA (A3085), and LiCl (L4408), were obtained from Sigma. Baf (S1413) and PF-00562271 (FAK inhibitor, S2672) were purchased from Selleckchem. TMR- dextran 70 kDa was obtained from ThermoFisher (D1818). PMA (1201) was purchased from TOCRIS.
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2

Cell Signaling Pathway Antibody Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total β-catenin antibody (1:1000) was purchased from Invitrogen (712700), glyceraldehyde-3-phosphate dehydrogenase antibody (1:1000) and FAK antibody (1:1000, 3285) were obtained from Cell Signaling Technologies, anti-ATP6V0a3 antibody (1:500) was obtained from Novus (nbp1-89333, 1:1000). CD63 antibody was obtained from Abcam.
Antibodies against Pak1 (ab131522), Ras (ab52939), GSK3 (ab93926, 1:4000), and secondary antibodies for immunostaining for cells (ab150120, ab150084, ab150117, ab150081) (1:300) were obtained from Abcam. Antibody against the FA protein Tes (HPA018123, 1:100) was obtained from Atlas antibodies. Secondary antibodies for immunostaining arrays (A-11001, A-11011 were obtained from Invitrogen). HRP-linked secondary antibodies 7076, 7074 at 1:5000 (Cell Signaling) were used for western blots and analyzed with an iBright Imaging system. EIPA (A3085), and LiCl (L4408), were obtained from Sigma. Baf (S1413) and PF-00562271 (FAK inhibitor, S2672) were purchased from Selleckchem. TMR-dextran 70 kDa was obtained from Thermo Fisher (D1818). PMA (1201) was purchased from TOCRIS.
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