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Necrosulphonamide

Manufactured by Merck Group
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Necrosulphonamide is a laboratory reagent used in various research applications. It is a sulfonamide compound that functions as a cell death inducer. The core function of Necrosulphonamide is to facilitate the study of cell death and apoptosis in biological systems.

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Lab products found in correlation

Flexbeads, by BD (2 mentions) Xtt 2 assay, by Roche (2 mentions) Milliplex immunoassay, by Merck Group (2 mentions) Gsk 872, by Merck Group (2 mentions)

2 protocols using necrosulphonamide

1

Investigating PDA Cell Line Responses

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The human PDA cell lines AsPC1, PANC1, and MIA PaCa-2 cells (gifts of Dafna Bar-Sagi, originally obtained from ATCC) were maintained in complete RPMI (RPMI 1640 with 10% heat-inactivated FBS, 2 mM L-glutamine, 1% Penicillin/Streptomycin). Cell lines were not authenticated. Cells were free of mycoplasma. In selected experiments, cells were treated with Gemcitabine (10–50µM), Nec-1s (50µM), a RIP3 inhibitor (GSK872; 6 µM), or a MLKL inhibitor (Necrosulphonamide, 1µM, both EMD Millipore, Billerica, MA). Cellular viability was determined by PI staining. Cellular proliferation was assessed using the XTT II assay according to the manufacturer’s protocol (Roche, Pleasanton, CA) and expressed as % proliferation compared to control. Inflammatory mediators in cell culture supernatant were measured using the Milliplex Immunoassay (Millipore, Billerica, MA). CXCL1 was additionally measured using Flexbeads (BD Biosciences) and ELISA (R&D Systems).
Seifert L., Werba G., Tiwari S., Giao Ly N.N., Alothman S., Alqunaibit D., Avanzi A., Barilla R., Daley D., Greco S.H., Torres-Hernandez A., Pergamo M., Ochi A., Zambirinis C.P., Pansari M., Rendon M., Tippens D., Hundeyin M., Mani V.R., Hajdu C., Engle D, & Miller G. (2016). The Necrosome Promotes Pancreas Oncogenesis via CXCL1 and Mincle Induced Immune Suppression. Nature, 532(7598), 245-249.
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2

Investigating PDA Cell Line Responses

Check if the same lab product or an alternative is used in the 5 most similar protocols
The human PDA cell lines AsPC1, PANC1, and MIA PaCa-2 cells (gifts of Dafna Bar-Sagi, originally obtained from ATCC) were maintained in complete RPMI (RPMI 1640 with 10% heat-inactivated FBS, 2 mM L-glutamine, 1% Penicillin/Streptomycin). Cell lines were not authenticated. Cells were free of mycoplasma. In selected experiments, cells were treated with Gemcitabine (10–50µM), Nec-1s (50µM), a RIP3 inhibitor (GSK872; 6 µM), or a MLKL inhibitor (Necrosulphonamide, 1µM, both EMD Millipore, Billerica, MA). Cellular viability was determined by PI staining. Cellular proliferation was assessed using the XTT II assay according to the manufacturer’s protocol (Roche, Pleasanton, CA) and expressed as % proliferation compared to control. Inflammatory mediators in cell culture supernatant were measured using the Milliplex Immunoassay (Millipore, Billerica, MA). CXCL1 was additionally measured using Flexbeads (BD Biosciences) and ELISA (R&D Systems).
Seifert L., Werba G., Tiwari S., Giao Ly N.N., Alothman S., Alqunaibit D., Avanzi A., Barilla R., Daley D., Greco S.H., Torres-Hernandez A., Pergamo M., Ochi A., Zambirinis C.P., Pansari M., Rendon M., Tippens D., Hundeyin M., Mani V.R., Hajdu C., Engle D, & Miller G. (2016). The Necrosome Promotes Pancreas Oncogenesis via CXCL1 and Mincle Induced Immune Suppression. Nature, 532(7598), 245-249.
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