Sh igf2bp3

Short hairpin RNA (shRNA) targeted GLI2, SYVN1, IGF2BP3, IGF2BP2, HNRNPA2B1, HNRNPD, HNRNPK, HNRNPM, HNRNPA1 (shGLI2, shSYVN1, shIGF2BP3, shIGF2BP2, shHNRNPA2B1, shHNRNPD, shHNRNPK, shHNRNPM, and shHNRNPA1) or scrambled oligonucleotides, purchased from GenePharma (Shanghai, China) were inserted into pGLVH1 vector. The shRNA sequences were provided in Table S1. The full length of SYVN1 or PPARα coding sequence was amplified and cloned into a pcDNA3.1 (Invitrogen, Carlsbad, CA, USA) vector (SYVN1, PPARα). Cells were transfected with the above plasmids using Lipofectamine 3000 transfection reagent (Invitrogen) according to manufacturer’s instructions. Lenti-Pac HIV Expression Packaging Mix and the lentiviral vectors (shSYVN1, shIGF2BP3, shGLI2) were used to transfect HEK293T cells for 48 hours and then lentiviral particles were harvested from the supernatant. Stable transfected cells were selected by two weeks puromycin treatment (2 μg/mL).