The cells were harvested at the indicated time points and washed twice with PBS/0.5% BSA at 4 °C to block the Fc receptors. The transduced CD34+ HPCs were stained with APC-conjugated anti-CD14 (clone: 61D3) or anti-CD11b (clone: ICRF44) antibodies (eBioscience, San Diego, CA). Mouse BM cells were stained with a PE-conjugated anti-CD33 antibody (clone: HIM3-4 eBioscience, CA, USA). Flow cytometry was performed on a C6 Flow Cytometer (BD Biosciences, Franklin Lakes, NJ). The data were analyzed using FlowJo software (Version 7.6, TreeStar, Ashland, OR).
Anti cd11b clone icrf44
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
Anti-CD11b (clone: ICRF44) is a monoclonal antibody that specifically binds to the CD11b antigen. CD11b is a cell surface receptor expressed on various immune cells, including monocytes, macrophages, and neutrophils. This antibody can be used to identify and study these cell populations in research applications.
Automatically generated - may contain errors
Lab products found in correlation
C6 flow cytometer, by BD (1 mentions)
Anti cd83 clone hb15e, by BD (1 mentions)
Anti cd86 clone it2, by BioLegend (1 mentions)
Anti cd14 clone m5e2, by BioLegend (1 mentions)
Anti hla dr clone l243, by BioLegend (1 mentions)
Anti cd1c clone l161, by BioLegend (1 mentions)
Zombie uv fixable viability dye, by BioLegend (1 mentions)
Facsdiva, by BD (1 mentions)
Lsrii flow cytometer, by BD (1 mentions)
Fortessa, by BD (1 mentions)
2 protocols using anti cd11b clone icrf44
1
Characterization of CD34+ HPCs
2
Comprehensive Multicolor Flow Cytometry Analysis
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Cells were analyzed by flow cytometry using the following anti-human antibodies: anti-CD14 (clone M5E2; BioLegend, London, UK), anti-CD86 (clone IT2.2; BioLegend), anti-CD83 (clone HB15e, BD Bioscience, Oxford, UK), anti-CD11c (clone 3.9; BioLegend), anti-CD1c (clone L161; BioLegend), anti-HLA-DR (clone, L243; BioLegend), anti-CD13 (clone WM15; BioLegend), anti-CD33 (clone P67.6; BioLegend), anti-CD141 (clone 1A4; BD Bioscience, Oxford, UK), and anti-CD11b (clone ICRF44; eBioscience). Dead cells were identified using fixable Viability dye Zombie UV (BioLegend, UK). The data were acquired on a LSRII flow cytometer or Fortessa (BD Bioscience) and analyzed using FACSDiva (BD Bioscience) or FlowJo software (Tree Star, Ashland, Oregon).
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