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Anti osteopontin

Manufactured by Merck Group
Sourced in Germany, United States

Anti-osteopontin is a laboratory reagent used for the detection and quantification of the glycoprotein osteopontin in various biological samples. Osteopontin is involved in a range of physiological and pathological processes, making it a potential biomarker. The anti-osteopontin reagent can be used in various analytical techniques, such as ELISA, Western blotting, and immunohistochemistry, to measure osteopontin levels in research and clinical settings.

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2 protocols using anti osteopontin

1

Quantifying Inflammatory Mediators

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Rabbit polyclonal antibodies that recognise MMP-1, MMP-9, IL-6, and TNFα were purchased from Acris Antibodies (GmbH, Germany), while anti-osteopontin was purchased from Millipore. The rabbit polyclonal antibody raised against actin and the secondary anti-rabbit antibody conjugated with alkaline phosphatase were purchased from Sigma-Aldrich Chem Co (St. Louis, MO, USA). All other chemicals used in the study were also purchased from Sigma-Aldrich.
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2

Western Blot, Immunofluorescence, and Immunohistochemistry Antibodies

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Antibodies anti-human proteins used for Western blot, immunofluorescence and immunohistochemistry were the mouse monoclonal anti-Nodal (ab55676, Abcam, Cambridge, UK), anti-MAP2 (MS-249-R7, ThermoFisher Scientific), anti-PARP1 (sc-8007, Santa Cruz Biotechnology, Dallas, TX, USA), anti-CD44v6 (33-6700, ThermoFisher Scientific), anti-CD31 (M0823, Dako, Agilent Technologies, Santa Clara, CA, USA), anti-Glypican-2 (sc-393824, Santa Cruz Biotechnology), anti-Nucleolin (39-6400, ThermoFisher Scientific), anti-β-catenin (sc-7963, Santa Cruz Biotechnology), anti-LRP6 (sc-25317, Santa Cruz Biotechnology), anti-p53 (sc-126, Santa Cruz Biotechnology), and the rabbit polyclonal anti-N-Cadherin (04-1126, Millipore), anti-Ki-67 (ab833, Abcam), anti-P-Akt and anti-Akt (9271 and 9272, Cell Signaling Technology Inc., Danvers, MA, USA), anti-CD114 (PA5-28988, ThermoFisher Scientific), anti-PES1 (HPA066670, Sigma, St Louis, MO, USA), anti-NPM1 (HPA011384, Sigma), anti-Osteopontin (AB1870, Millipore).
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