Oocyte preparation was as reported (Kim et al., 2015) . RNA from oocytes was isolated using the SuperPrep Cell Lysis & RT Kit for real-time PCR (TOYOBO, Osaka, Japan). PCR was performed on the Agilent AriaMx Real-Time PCR System. Primers were in Supplement Table S2. The relative number of transcripts was calculated by the cycle threshold method as described by Agilent using the AtiaMx Real-Time PCR System Software and normalized to the endogenous reference (β-actin). The relative amount of target gene expression for each sample was calculated and plotted as the mean ± SD.
Superprep cell lysis rt kit
Manufactured by Toyobo
Sourced in Japan
The SuperPrep Cell Lysis & RT Kit is a laboratory equipment product designed for cell lysis and reverse transcription. It provides a solution for the preparation of RNA samples from various cell types. The core function of this kit is to facilitate the extraction and purification of high-quality RNA from cells, enabling downstream molecular biology applications.
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Lab products found in correlation
2 protocols using superprep cell lysis rt kit
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Oocyte RNA Expression Analysis
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Oocyte RNA Expression Analysis
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Oocyte preparation was as reported (Kim et al., 2015) . RNA from oocytes was isolated using the SuperPrep Cell Lysis & RT Kit for real-time PCR (TOYOBO, Osaka, Japan). PCR was performed on the Agilent AriaMx Real-Time PCR System. Primers were in Supplement Table S2. The relative number of transcripts was calculated by the cycle threshold method as described by Agilent using the AtiaMx Real-Time PCR System Software and normalized to the endogenous reference (β-actin). The relative amount of target gene expression for each sample was calculated and plotted as the mean ± SD.
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