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Taqman amplicor hiv 1 monitor

Manufactured by Roche

The TaqMan® AMPLICOR HIV-1 MONITOR is a laboratory equipment product designed for the quantitative detection of Human Immunodeficiency Virus type 1 (HIV-1) ribonucleic acid (RNA) in human plasma or serum samples. It utilizes real-time reverse transcription-polymerase chain reaction (RT-PCR) technology to measure the viral load of HIV-1.

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2 protocols using taqman amplicor hiv 1 monitor

1

Quantification of Plasma HIV RNA

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TaqMan® AMPLICOR HIV-1 MONITOR (Roche Molecular Systems), an advanced molecular technique, was used for the quantification of HIV-1 RNA in plasma collected by EDTA tubes containing plasma separator gel from each study participant. Then, the amount of circulating HIV was measured and reported as HIV RNA copies/mL of plasma by well-trained laboratory technologists.
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2

Comprehensive Blood and Parasite Analysis

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About 5ml of blood sample was collected following standard operating procedures (SOPs) for complete blood cell count (CBC) and viral load determination. CBC parameters were analyzed using Sysmex KX21 hematology analyzer in accordance with the manufacturer’s instructions by well experienced hematologist. Furthermore, HIV viral load was determined directly by an advanced molecular technique using TAQMAN® AMPLICOR HIV-1 MONITOR (Roche Molecular Systems) according to the manufacturers instruction by well-trained laboratory technologists. For intestinal parasite examination, test tub flotation concentration procedures were done for the detection of protozoan cysts, helminthic ova, and larvae in addition to using the direct wet mount as per the standard protocol.
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