Rat hippocampal tissues were collected 24 h after reperfusion. Protein samples (40–50 μg in each lane) were loaded onto SDS PAGE gels for electrophoresis. Proteins were transferred to polyvinylidene difluoride membranes and then blocked in 5% dried milk at room temperature. Primary antibodies were added and incubated at 4 °C overnight. H3K27me3 (mAbcam6002) and histone H3 (mAbcam10799) were purchased from Abcam (Shanghai, China), and β-actin (ab179467) and caspase-3 (19677-1-AP) were purchased from Proteintech (Wuhan, China). Then, the membrane was incubated with the secondary antibody (dilution 1:5000) at 37 °C for 2 h. The protein bands were detected using a chemiluminescent substrate kit (Merck Millipore, USA) according to the manufacturer’s recommendations. Band intensities were quantified using Quantity One software.
β actin ab179467
Manufactured by Proteintech
Sourced in China
β-actin (ab179467) is a protein that is involved in various cellular processes, including cell motility, structure, and integrity. It is a widely used loading control and reference gene in molecular biology and cell biology experiments.
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Lab products found in correlation
2 protocols using β actin ab179467
1
Rat Hippocampal Protein Expression Analysis
2
Histone Methylation in Rat Hippocampus
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Rat hippocampal tissues were collected 24 hours after reperfusion. Protein samples (40-50 µg in each lane) were loaded onto SDS PAGE gels for electrophoresis. Proteins were transferred to polyvinylidene di uoride membranes and then blocked in 5% dried milk at room temperature. Primary antibodies were added and incubated at 4°C overnight. H3K27me3 (mAbcam6002) and histone H3 (mAbcam10799) were purchased from Abcam (Shanghai, China), and β-actin (ab179467) and caspase-3 (19677-1-AP) were purchased from Proteintech (Wuhan, China). Then, the membrane was incubated with the secondary antibody (dilution 1:5000) at 37°C for 2 h. The protein bands were detected using a chemiluminescent substrate kit (Merck Millipore, USA) according to the manufacturer's recommendations. Band intensities were quanti ed using Quantity One software.
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