Anti human prl 1

To assess specific gene expression of PD-MSCs PRL-1 and cirrhotic liver tissues, samples were lysed in protein lysis buffer (Sigma-Aldrich). The protein lysates were loaded on sodium dodecyl sulfate polyacrylamide gels, and separated proteins were transferred to PVDF membrane. The following primary antibodies were used: antihuman PRL-1, anti-Oct4 (1:1,000; all from Abcam, Cambridge, UK), anti-albumin, anti-HLA-G (4h84) (1:1,000; all from Novus Biologicals, Littleton, CO, USA), anti-ATP5B (1:200; Santa Cruz Biotechnology, Dallas, TX, USA), anti-ROCK1, anti-RhoA, anti-CDK4, anti-cyclin D1, and mitochondrial marker antibody sampler kit (1:1000; all from Cell Signaling Technology, Denvers, MA, USA). The loading control was anti-GAPDH (1:3,000; AbFrontier, Seoul, Korea). The following secondary antibodies were used: anti-mouse IgG (1:5,000; Bio-Rad, Hercules, CA, USA) and anti-rabbit IgG (1:10,000; Bio-Rad). The bands were detected using ECL reagent (Bio-Rad).

To assess the speci c gene expression of PD-MSCs PRL-1 and cirrhotic liver tissues, samples were lysed in protein lysis buffer (Sigma-Aldrich). The protein lysates were loaded on sodium dodecyl sulfate polyacrylamide gels, and separated proteins were transferred to PVDF membranes. The following primary antibodies were used: anti-human PRL-1, anti-Oct4 (1:1,000; all from Abcam, Cambridge, UK), antialbumin, anti-HLA-G (4h84) (1:1,000; all from Novus Biologicals, Littleton, CO, USA), anti-ATP5B (1:200; Santa Cruz Biotechnology, Dallas, TX, USA), anti-ROCK1, anti-RhoA, anti-CDK4, anti-cyclin D1, and mitochondrial marker antibody sampler kit (1:1000; all from Cell Signaling Technology, Denvers, MA, USA). The loading control was anti-GAPDH (1:3,000; AbFrontier, Seoul, Korea). The following secondary antibodies were used: anti-mouse IgG (1:5,000; Bio-Rad, Hercules, CA, USA) and anti-rabbit IgG (1:10,000; Bio-Rad). The bands were detected using ECL reagent (Bio-Rad).