Hplc grade acetonitrile acn and water

All used abbreviations of PFAAs are according to Buck et al. (2011) . Target PFAAs included 11 PFCAs (PFBA, PFPeA, PFHxA, PFHpA, PFNA, PFOA, PFDA, PFUnDA, PFDoDA, PFTrDA and PFTeDA) and 4 PFSAs (PFBS, PFHxS, PFOS and PFDS). Isotopically mass-labelled internal standards (ISTDs) were purchased by
Wellington Laboratories (Guelph, Canada) and comprised 13 C4-PFBA, [1,2-13 C2]PFHxA, [1,2,3,4-13 C4]PFOA, [1,2,3,4,5-13 C5]PFNA, [1,2-13 C2]PFDA, [1,2-13 C2]PFUnDA, 1,2[ 13 C2]PFDoDA, 18 O2-PFHxS and [1,2,3,4-13 C4]PFOS. HPLC grade Acetonitrile (ACN) and water (VWR International, Leuven, Belgium) were used.

Mussel (Mytilus edulis) byssus was kindly provided by Fastnet Mussels (Gearhies, Bantry, Co. Cork). ProtoGel®, ProtoGel® Resolving Buffer (4×), ProtoGel® Stacking Buffer (4×), Tris-Glycine-SDS-PAGE Buffer (10×) and Protein Loading Buffer Blue (2×) were obtained from National Diagnostics (Atlanta, USA). Quick Start Bradford 1 × Dye Reagent® was supplied by Bio-Rad (Dublin, Ireland). Bovine lung was kindly provided by Gaelic Meats and Livestock Ltd. (Limerick, Ireland), and H-Gly-Pro-AMC and Diprotin A were obtained from Bachem (Bubendorf, Switzerland). HPLC-grade acetonitrile (ACN) and water were obtained from VWR (Dublin, Ireland). Brewers Clarex® (Aspergillus niger derived prolyl endoproteinase (An-PEP) specific activity: 37 × 10 -3 U/mg) was supplied as a gift from Dutch State Mines (DSM, Heerlen, Netherlands), Corolase® PP was provided by AB Enzymes (Darmstadt, Germany), Alcalase® 2.4 L and Flavourzyme® 500 L were obtained from Novozymes A/S (Bagsvaerd, Denmark), Promod 144MG was kindly provided by Biocatalysts Ltd (Parc Nantgarw, Wales, UK). All other reagents, including collagenase from Clostridium histolyticum (Type 1), were obtained from Sigma Chemical Company Ltd. (Gillingham, UK).