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Sigmaplot 12.5 enzyme kinetics module

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SigmaPlot 12.5 Enzyme Kinetics Module is a software tool designed for analyzing and visualizing enzyme kinetics data. It provides tools for fitting enzyme kinetic models, calculating kinetic parameters, and creating publication-quality graphs.

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Lab products found in correlation

Spss statistics ver 22, by IBM (1 mentions)

2 protocols using sigmaplot 12.5 enzyme kinetics module

1

Enzyme Kinetic Parameter Determination

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Michaelis constant (K m ), maximum velocity (V max ), and intrinsic clearance (CL int = V max /K m ) values were calculated using SigmaPlot 12.5 Enzyme Kinetics Module (Systat Software Inc., Chicago, USA). Each value was obtained in triplicate and expressed as mean ± standard deviation. All assays and measurements were performed in triplicate using a single S-9 preparation. Statistical variance in protein expression and kinetic parameters was analyzed using IBM SPSS Statistics Ver. 22 (International Business Machines, Armonk, NY, USA). Differences or correlations with P < 0.05 were considered significant.
Hishinuma E., Narita Y., Rico E.M.G., Ueda A., Obuchi K., Tanaka Y., Saito S., Tadaka S., Kinoshita K., Maekawa M., Mano N., Nakayoshi T., Oda A., Hirasawa N, & Hiratsuka M. (2023). Functional Characterization of 12 Dihydropyrimidinase Allelic Variants in Japanese Individuals for the Prediction of 5-Fluorouracil Treatment-Related Toxicity. Drug metabolism and disposition: the biological fate of chemicals, 51(2).
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2

Enzyme Kinetics Analysis of DPD Expression

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Kinetic data were analyzed using SigmaPlot 12.5 Enzyme Kinetics Module (Systat Software Inc., Chicago, IL, United States); Michaelis constant (Km), maximum velocity (Vmax), and intrinsic clearance (CLint = Vmax/Km) values were determined using the average DPD expression values obtained in triplicate and are expressed as mean ± standard deviation. Statistical variance in protein expression and kinetic parameters was analyzed using Dunnett’s t-test, Dunnett’s T3 test, or the Kruskal-Wallis method (IBM SPSS Statistics Ver. 22, International Business Machines, Armonk, NY, United States). Statistical significance was set at p < 0.05.
Hishinuma E., Narita Y., Obuchi K., Ueda A., Saito S., Tadaka S., Kinoshita K., Maekawa M., Mano N., Hirasawa N, & Hiratsuka M. (2022). Importance of Rare DPYD Genetic Polymorphisms for 5-Fluorouracil Therapy in the Japanese Population. Frontiers in Pharmacology, 13, 930470.
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