The following cells and reagents were used in this study: human embryonic kidney 293T cells (Shanghai Laboratory Animal Center, Chinese Academy of Sciences); SH-SY5Y cells (Institute of Basic Medical Sciences, Peking Union Medical College); MPP + , 4-hydroxy tamoxifen (4HT), ploybrene, puromycin, and hygromycin B (Sigma-Aldrich, Seelze, Germany); fetal bovine serum (FBS), primary culture FBS, DMEM high-glucose culture medium, DMEM high-glucose culture medium with HEPES, trypsin, PBS, and double-antibody penicillinstreptomycin (Wisent Biotechnology Nanjing Co., Ltd., Ninjang, China); Lipofectamine 2000 transfection reagent kits (Invitrogen, Carlsbad, CA, USA); Effectene Transfection Reagent (QIAGEN, Hilden, Germany); centrifuge (centrifuge 5415D), refrigerated centrifuge (5475R), and ultraviolet spectrophotometer (Eppendorf, Hamburg, Germany); DNA electrophoresis apparatus (Danyang No. 1 Wireless Factory, Danyang, China); DNA electrophoresis tank (Model DYCP-32 electrophoresis tank) (Beijing Bayi Instrument Plant, Beijing, China); Western blot electrophoresis equipment (Bio-Rad Company, Hercules, CA, USA).
Western blot electrophoresis equipment
Manufactured by Bio-Rad
Sourced in China, United States
Western blot electrophoresis equipment is a laboratory instrument used for the separation and analysis of proteins. It enables the separation of proteins based on their molecular weight through the process of electrophoresis. This equipment is a core tool in the field of protein analysis and is widely used in various research and diagnostic applications.
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2 protocols using western blot electrophoresis equipment
1
Detailed Experimental Protocols for Cell Lines
2
Antibody-based Protein Analysis Protocol
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The primary antibodies used in the experiment were purchased from BD, USA, and the secondary antibodies were purchased from Invitrogen, USA. PcDNA3.1 carriers are provided by Shanghai Gemal Biotechnology Co., Ltd.,Shanghai, China. Lipofectamine™ 2000 transfection reagent was purchased from BD, USA. The RT-PCR instrument was purchased from BD, USA, and the HBS-1096B microplate was purchased from Nanjing Detie Experimental Equipment Co., Ltd. The Western blot electrophoresis equipment was purchased from Bio-Rad, USA.
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