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Shep 1

Manufactured by American Type Culture Collection
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SHEP-1 is a cell line derived from a human epithelial cell. It is maintained in culture media and can be used for various research applications.

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Lab products found in correlation

Sh sy5y, by American Type Culture Collection (3 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Sk n dz, by American Type Culture Collection (3 mentions) Sk n as, by American Type Culture Collection (3 mentions) Be 2 c, by American Type Culture Collection (2 mentions) Sk n f1, by American Type Culture Collection (2 mentions) Imr 32, by American Type Culture Collection (2 mentions) Dmem f12, by Thermo Fisher Scientific (2 mentions) Rpmi 1640 media, by Thermo Fisher Scientific (1 mentions) Complete rpmi medium, by Thermo Fisher Scientific (1 mentions) Igrov 1, by Merck Group (1 mentions) Sk n as, by Merck Group (1 mentions) Complete dmem media, by Thermo Fisher Scientific (1 mentions) Be 2 c, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) N formyl l methionyl l leucyl l phenylalanine fmlp, by Merck Group (1 mentions) Rpmi 1640 medium, by Merck Group (1 mentions) Sk n be 2, by American Type Culture Collection (1 mentions) Sk n fi, by American Type Culture Collection (1 mentions)

5 protocols using shep 1

1

Culturing Ovarian and Neuroblastoma Cell Lines

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SKOV-3
(ATCC, HTB77), OVCAR-3 (ATCC, HTB-161),
OVCAR-8 (inherited from Laurent Brad’s previous laboratory),
and CAOV-3 (ATCC, HTB75) ovarian cancer cells were grown in complete
DMEM media (Gibco, 11965). IGROV-1 (Sigma, SCC203) and 2008 (kindly
provided by Dr. François X. Claret, University of Texas M.D.
Anderson Cancer Center) ovarian cancer cells were grown in complete
RPMI medium (Gibco, 22400). ES2 (ATCC, CRL-1978) was grown in McCoy’s
5A complete medium (ATCC, 30-2007). BE(2)C (ATCC, CRL-2268), SH-EP1
(ATCC, CRL-2269), SH-SY5Y (ATCC, CRL-2266), KELLY (Sigma, 92110411),
SK-N-AS (Sigma, 94092302), and LAN-5 (COG, http://www.cogcell.org) neuroblastoma
cell-lines were maintained in RPMI1640 media (Gibco, 11875) supplemented
with 10% heat-inactivated FBS. TH-MYCN+/+ cells were derived by mechanical
dissociation of tumors obtained from TH-MYCN homozygous mice51 (link)−53 (link) and were maintained in RPMI1640 media (Gibco, 11875) supplemented
with 20% heat-inactivated FBS, 10–5 mM 2-mercaptoethanol, 1
mM sodium pyruvate, and 1× nonessential amino acids (Gibco, 11140076).
Khazan N., Kim K.K., Hansen J.N., Singh N.A., Moore T., Snyder C.W., Pandita R., Strawderman M., Fujihara M., Takamura Y., Jian Y., Battaglia N., Yano N., Teramoto Y., Arnold L.A., Hopson R., Kishor K., Nayak S., Ojha D., Sharon A., Ashton J.M., Wang J., Milano M.T., Miyamoto H., Linehan D.C., Gerber S.A., Kawar N., Singh A.P., Tabdanov E.D., Dokholyan N.V., Kakuta H., Jurutka P.W., Schor N.F., Rowswell-Turner R.B., Singh R.K, & Moore R.G. (2022). Identification of a Vitamin-D Receptor Antagonist, MeTC7, which Inhibits the Growth of Xenograft and Transgenic Tumors In Vivo. Journal of Medicinal Chemistry, 65(8), 6039-6055.
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2

Culturing Human Neuroblastoma Cell Lines

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The human neuroblastoma cell lines BE(2)-C, SK-N-DZ, SK-N-F1, SHEP1 and IMR32 were purchased from ATCC (Rockville, MD, USA), and all cell lines except BE(2)-C were cultured in complete medium containing DMEM (Life Technologies, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (Invitrogen) and 1% penicillin/streptomycin (Life Technologies, Grand Island, NY, USA). BE(2)-C cells were cultured in DMEM/F-12 complete medium. All cells were maintained at 37°C in a humidified incubator containing 5% CO2.
Zhang D., Wang F., Pang Y., Ke X.X., Zhu S., Zhao E., Zhang K., Chen L, & Cui H. (2017). Down-regulation of CHERP inhibits neuroblastoma cell proliferation and induces apoptosis through ER stress induction. Oncotarget, 8(46), 80956-80970.
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3

Characterization of Human Neuroblastoma Cell Lines

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The human neuroblastoma cell lines (SK-N-FI, SHEP-1, SK-N-BE (2), SK-N-SH, SK-N-AS, SH-SY5Y, SK-N-DZ) were purchased from the American Type Culture Collection (ATCC) (Table 1). The cells were cultured in humidified air with 5 % CO2 at 37 °C in RPMI-1640 medium with L-glutamine and sodium bicarbonate (R8758, Sigma-Aldrich Chemie GmbH, Steinheim, Germany), and supplemented with 10 % FBS. N-Formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP) was purchased from Sigma-Aldrich (F3506, St. Louis, MO, USA) and Cyclosporin H from Enzo Life Sciences (ALX-380-286, Farmingdale, NY, USA).

Characteristics of the cell lines used in this study

SK-N-FISHEP-1SK-N-BE(2)SK-N-SHSK-N-ASSH-SY5YSK-N-DZ
MYCN amplification--+---+
MDR phenotype+-+++--
1p deletion--+-+--
11q deletion----+-+

MDR multiple drug resistance

Snapkov I., Öqvist C.O., Figenschau Y., Kogner P., Johnsen J.I, & Sveinbjørnsson B. (2016). The role of formyl peptide receptor 1 (FPR1) in neuroblastoma tumorigenesis. BMC Cancer, 16, 490.
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4

Neuroblastoma Cell Line Characterization

Cited 3 times
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IMR-32, SH-SY5Y, SH-EP1, and SK-N-AS human neuroblastoma cells were obtained from the American Type Culture Collection (Rockville, MD). SH-EP1 cells were transfected to effect overexpression (p75OE cells) or mock transfected (OE Ctrl) as we have previously described [5 (link), 10 (link), 15 (link)].
Yang Y.P., Wang S., Li X, & Schor N.F. (2015). Cell Line-Dependent Variability of Coordinate Expression of p75NTR and CRABP1 and Modulation of Effects of Fenretinide on Neuroblastoma Cells. Oxidative Medicine and Cellular Longevity, 2016, 7568287.
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5

Culturing Human Neuroblastoma Cell Lines

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The human neuroblastoma cell lines SK-N-AS, BE(2 (link))C, SK-N-DZ, SK-N-F1 and SHEP1 were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA). The BE(2 (link))C cells were cultured in a 1:1 mixture of Dulbecco's modified Eagle's medium (DMEM) and Ham's nutrient mixture F12 (DMEM/F12; Thermo Fisher Scientific, Inc., Waltham, MA, USA) with 10% fetal bovine serum (FBS; Thermo Fisher Scientific, Inc.) and 1% penicillin/streptomycin (P/S). SK-N-AS, SK-N-DZ, SK-N-F1 and SHEP1 cells were cultured in DMEM (Thermo Fisher Scientific, Inc.) with 10% FBS and 1% P/S. The 293FT cell line (ATCC) was cultured with DMEM (Gibco; Thermo Fisher Scientific, Inc.) containing 1% glutamine, glycine and pyruvate. All cells were incubated at 37°C in an incubator with 5% CO2.
Pang Y., Pan L., Zhang Y, & Liu G. (2019). TP53BP2 decreases cell proliferation and induces autophagy in neuroblastoma cell lines. Oncology Letters, 17(6), 4976-4984.
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