Hif 1α gtx127309

Manufactured by GeneTex

HIF-1α (GTX127309) is a lab equipment product from GeneTex. It is a protein that plays a key role in the cellular response to hypoxia, or low oxygen levels. This product can be used for research purposes, but a detailed description of its intended use cannot be provided in an unbiased and factual manner.

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Lab products found in correlation

2 protocols using hif 1α gtx127309

Antibody Characterization for Western Blot Analysis

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Antibodies used for western blot analysis are as follows: HIF-1α (GTX127309) from GeneTex; HIF-1α (NB100-479) and HIF-2α (NB100-122) from Novus Biologicals; HIF-2α (#7096), pVHL (#2738), FASN (#3180) and ubiquitin (#3936) from Cell Signaling; Elongin C (sc-135895), CUL2 (sc-166506), ARNT (sc-5580), SREBP1 (sc-366), and normal mouse and rabbit IgG (sc-2025 and sc-2027 respectively) from Santa Cruz Biotechnology; CAD (ab40800), RNA polymerase II large subunit CTD (ab5408), and CA9 (ab107257) from Abcam; β-actin (A5441) and anti-Flag M2 (F1804) from Sigma-Aldrich; p65 (610869) from Becton Dickinson (BD) and REDD1 (A500-001A) from Bethyl Laboratories. For immunofluorescence, antibodies against pVHL (BD, 556347) or FASN (#3180) were used. For immunoprecipitation, antibodies against pVHL (BD, 556347), FASN (Santa Cruz Biotechnology, sc-55580) or CAD (Abcam, ab40800) were used.

Sun W., Kato H., Kitajima S., Lee K.L., Gradin K., Okamoto T, & Poellinger L. (2017). Interaction between von Hippel-Lindau Protein and Fatty Acid Synthase Modulates Hypoxia Target Gene Expression. Scientific Reports, 7, 7190.

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Immunoblotting Antibody Specifications

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Antibodies for immunoblotting such as p‐AMPKα (#2535), AMPKα (#5831), PDK1 (#3820), HIF1α (#3716), hexokinase 2 (#2867), LDHA (#3582), PFKP (#8164), PGAM1 (#12098), PDH (#3205), p‐Threonine/Tyrosine (#9381), p‐S6K (#9234), S6K (#2708), p‐ERK (#4370), ERK (#4695), p‐4E‐BP1 (Thr37/46, #2855), p‐4E‐BP1 (Ser65, #9451), 4E‐BP1 (#9644), mouse antirabbit IgG mAb (#5127), and antirabbit IgG (#7074) were from Cell Signaling Technology (Danvers, MA, USA). HIF1α (hydroxyl P564) (ab72777) and PHD3 (ab30782) were purchased from Abcam (Cambridge, MA, USA). HIF1α (hydroxyl P402) (07‐1585) was purchased from Merck (Darmstadt, Germany). GAPDH (sc‐25778) was purchased from Santa Cruz Biotechnology (Paso Robles, CA, USA). Actin (GTX109639) was purchased from GeneTex International (Hsinchu, Taiwan). Antibodies for immunoprecipitation such as HIF1α (#36169), rabbit mAb IgG (#3900), and p‐AMPKα (#2535) were purchased from Cell Signaling Technology. AMPKα1 (sc‐398861) was purchased from Santa Cruz Biotechnology. Antibodies for IHC such as p‐AMPKα (GTX52341) and HIF1α (GTX127309) were purchased from GeneTex International.

Tseng H., Zeng Y., Lin Y.J., Huang J., Lin C., Lee M., Yang F., Fang T., Mar A, & Su J. (2022). A novel AMPK activator shows therapeutic potential in hepatocellular carcinoma by suppressing HIF1α‐mediated aerobic glycolysis. Molecular Oncology, 16(11), 2274-2294.

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