Thp 1 tib 202

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THP-1 (TIB-202) is a human acute monocytic leukemia cell line derived from the peripheral blood of a 1-year-old male with acute monocytic leukemia. This cell line is commonly used in research for the study of monocyte and macrophage biology.

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Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (7 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (3 mentions) Jurkat tib 152, by American Type Culture Collection (3 mentions) Anti cd14 conjugated magnetic microbeads, by Miltenyi Biotec (2 mentions) Raw264.7 tib 71, by American Type Culture Collection (2 mentions) Dulbecco modified eagle medium (dmem), by American Type Culture Collection (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Phorbol myristate acetate (pma), by Merck Group (2 mentions) K562 ccl 243, by American Type Culture Collection (2 mentions) Caco 2 htb 37, by American Type Culture Collection (2 mentions) Mv4 11, by American Type Culture Collection (2 mentions) Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (2 mentions) Fbs 10, by Merck Group (2 mentions) Antibiotic antimycotic, by Thermo Fisher Scientific (2 mentions) L glutamine, by Thermo Fisher Scientific (2 mentions) Hl 60 ccl 240, by American Type Culture Collection (2 mentions) Lsr 2, by BD (2 mentions) Facscanto, by BD (2 mentions) In situ cell death detection kit, by Roche (2 mentions) Cytotoxicity detection kit, by Roche (2 mentions)

Close spelling variants of this product

THP-1 (1072 citations) TIB-202 (277 citations) TIB-202TM (23 citations) THP-1 ATCC® TIB-202™) (18 citations) THP-1 (TIB-202) cells (7 citations) THP-1 cells (ATCC® TIB-202) (4 citations) Human THP-1 (ATCC TIB-202) (2 citations)

38 protocols using thp 1 tib 202

Cell Line Provenance Verification

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K562 (CCL-243), 293T (CRL-3216), HeLa (CCL-2), A375 (CRL-1619), A2058 (CRL-11147), SH4 (CRL-7724), MDA-MB-435S (HTB-129), SK-MEL-5 (HTB-70), SK-MEL-30 (HTB-63) and THP1 (TIB-202) cell lines were obtained from the American Type Culture Collection (ATCC). UACC-62, UACC-257 and LOX-IMVI cells were obtained from the Frederick Cancer Division of Cancer Treatment and Diagnosis (DCTD) Tumor Cell Line Repository. All cell lines were re-authenticated by STR profiling at ATCC before submission of the manuscript and compared to ATCC and Cellosaurus (ExPASy) STR profiles in 2020, with the exception of THP1 (TIB-202) and U937 (CRL-1593.2), which were purchased from ATCC for the experiments. Cells lines from the PRISM collection were obtained from The PRISM Lab (Broad Institute) and were not further re-authenticated. MDA-MB-435S cells were previously assumed to be ductal carcinoma cells and recent gene expression analysis assigned them to the melanoma lineage (ATCC).

Skinner O.S., Blanco-Fernández J., Goodman R.P., Kawakami A., Shen H., Kemény L.V., Joesch-Cohen L., Rees M.G., Roth J.A., Fisher D.E., Mootha V.K, & Jourdain A.A. (2023). Salvage of ribose from uridine or RNA supports glycolysis in nutrient-limited conditions. Nature Metabolism, 5(5), 765-776.

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Cultivation and Characterization of Cell Lines

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The following cell types, HEK293T (CRL-3216), Vero (CCL-81), Hs 729T (HTB-153), THP-1 (TIB-202) and T84 (CCL-248), were obtained from the ATCC (Manassas, VA, USA). HEK293 cells were purchased from Microbix Biosystems Inc (Mississauga, ON, Canada), and 911 cells were already described [23 (link)]. HEK293T-hACE2 cells were already described [24 (link)]. All the cell lines were grown in the recommended medium supplemented with 15% of fetal bovine serum (Natocor, Cordoba, Argentina), 2 mM glutamine, 100 U/mL penicillin and 100 μg/mL streptomycin and maintained in a 37 °C atmosphere containing 5% CO₂. For the HEK293T and HEK293T-hACE2 cell cultures, non-essential amino acids (1X final concentration) were added. Immature dendritic cells (iDC) were generated from THP-1 monocytes as previously described [25 (link)]. To induce differentiation, THP-1 monocytes were cultured during 5 days in a RPMI-1640 medium (Gibco, Whaltman, MD, USA), 2-mercaptoethanol (0.05 mM final concentration; Gibco, Whaltman, MD, USA) and fetal bovine serum (10%), adding rhIL-4 (100 ng = 1500 IU/mL; Peprotech, Cranbury, NJ, USA) and rhGM-CSF (100 ng = 1500 IU/mL; Peprotech, Cranbury, NJ, USA). The acquired properties of iDCs were analyzed under a microscope. A medium exchange was performed every 2 days with a fresh cytokine-supplemented medium.

López M.V., Vinzón S.E., Cafferata E.G., Núñez F.J., Soto A., Sanchez-Lamas M., Afonso M.J., Aguilar-Cortes D., Ríos G.D., Maricato J.T., T. Braconi C., B. Silveira V., M. Andrad T., C. S. Bonetti T., Ramos Janini L.M., Girão M.J., Llera A.S., Gomez K.A., Ortega H.H., Berguer P.M, & Podhajcer O.L. (2021). A Single Dose of a Hybrid hAdV5-Based Anti-COVID-19 Vaccine Induces a Long-Lasting Immune Response and Broad Coverage against VOC. Vaccines, 9(10), 1106.

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Isolation and Purification of Human Monocytes

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Monocytes from healthy blood donors were isolated and purified from buffy coats, following a protocol approved by the Institutional Review Board of Shanghai Jiao Tong University. Briefly, human peripheral blood mononuclear cells (PBMCs) were separated by centrifugation on Ficoll-Paque gradient. Monocytes were then isolated from PBMCs by positive sorting using anti-CD14-conjugated magnetic microbeads (Miltenyi, Germany). Monocyte purity was greater than 90% as assessed by flow cytometry. Mouse peritoneal macrophages were prepared from WT or knockout mice with C57BL/6 background as described [41 ]. Human monocytic leukemia cell line THP-1 (TIB-202) was purchased from ATCC (Manassas, VA). Cells were maintained in RPMI 1640 supplemented with 10% FBS, 1% streptomycin-penicillin and 10 μM β-mercaptoethanol. All cell cultures were kept in a humidified atmosphere with 5% CO₂ at 37°C.

Sun L., Zhu Z., Cheng N., Yan Q, & Ye R.D. (2014). Serum amyloid A induces interleukin-33 expression through an IRF7-dependent pathway. European journal of immunology, 44(7), 2153-2164.

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Isolation and culture of human and mouse macrophages

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Monocytes were purified from buffy coat obtained from healthy blood donors, following a protocol approved by the Institutional Review Board at Shanghai Jiao Tong University. Briefly, human peripheral blood mononuclear cells (PBMCs) were separated by standard gradient centrifugation on Ficoll-Paque gradient. Monocytes were then isolated from PBMCs by positive sorting using anti-CD14-conjugated magnetic microbeads (Miltenyi, Germany). Monocyte purity was greater than 90% as assessed by flow cytometry (data not shown). Mouse bone marrow-derived macrophages (BMDMs) were prepared from bone marrow cells isolated from WT C57BL/6 mice or knockout mice as described previously (34 ). Human monocytic leukemia cell line THP-1 (TIB-202) and mouse macrophage cell line RAW264.7 (TIB-71) was purchased from ATCC (Manassas, VA). The cells were maintained in RPMI 1640 supplemented with 10% FBS, 1% streptomycin-penicillin and 10 μM β-mercaptoethanol. All cell cultures were kept at 37 °C in a humidified atmosphere with 5% CO₂.

Sun L., Zhou H., Zhu Z., Yan Q., Wang L., Liang Q, & Ye R.D. (2015). Ex vivo and in vitro effect of serum amyloid A in the induction of macrophage M2 markers and efferocytosis of apoptotic neutrophils. Journal of immunology (Baltimore, Md. : 1950), 194(10), 4891-4900.

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Vascular Smooth Muscle Cell Protocols

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The mouse vascular smooth muscle (MOVAS) cell line (CRL-2797TM) and human aortic smooth muscle cell (HASMC) strain (PCS-100-012TM) were from American Type Culture Collection (ATCC, Rockville, MD, USA). The human monocytic cell line THP-1 (TIB-202™) was also from ATCC and was kindly offered by Dr. X. Liu (Neurosurgery Department, Tongji Hospital). For cell culture and treatment details, please see the Supplementary Materials.

Li Q., Zhang C., Shi J., Yang Y., Xing X., Wang Y., Zhan X., Wang L., Xu G, & He F. (2022). High-Phosphate-Stimulated Macrophage-Derived Exosomes Promote Vascular Calcification via let-7b-5p/TGFBR1 Axis in Chronic Kidney Disease. Cells, 12(1), 161.

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Culturing and Isolating Diverse Cell Lines

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HEK293T (CRL-11268; ATCC), HeLa (CRM-CCL-2; ATCC), and TZM-bl (PTA-5659; ATCC) cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; HyClone) containing 10% heat-inactivated fetal bovine serum (FBS, 04-001-1; Biological Industries) and penicillin/streptomycin. H9 (HTB-176; ATCC), Jurkat (TIB-152; ATCC), and THP1 (TIB-202; ATCC) cells were purchased from the ATCC and maintained in Roswell Park Memorial Institute 1640 (RPMI-1640) medium (HyClone) with 10% FBS and penicillin/streptomycin. The peripheral blood mononuclear cells (PBMCs) were isolated through Ficoll gradient centrifugation, and the CD4⁺ T lymphocytes were then purified from the PBMCs with anti-CD4-specific antibody-coated microbeads (Miltenyi Biotec, Germany) according to the manufacturer’s instructions. CD4⁺ T lymphocytes were maintained in RPMI-1640 medium (HyClone) with 10% FBS and penicillin/streptomycin.

Gao W., Rui Y., Li G., Zhai C., Su J., Liu H., Zheng W., Zheng B., Zhang W., Yang Y., Hua S, & Yu X. (2021). Specific Deubiquitinating Enzymes Promote Host Restriction Factors Against HIV/SIV Viruses. Frontiers in Immunology, 12, 740713.

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Cell Culture Protocols for Tumor and Immune Cell Lines

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Jurkat (TIB-152) human T cell lymphoma and THP-1 (TIB202) human acute monocytic lymphoma cell lines were obtained from ATCC (Manassas, VA). The BV-2 murine microglia cell line was a generous gift of Prof. Rosario Donato (Università degli Studi di Perugia, Perugia, Italy) [9 (link)]. MH-S murine alveolar macrophage cell line originally obtained from ATCC was kindly provided by Dr. Dolores Solis (Madrid, Spain). The cell lines were cultured in RPMI medium containing 10% (v/v) fetal bovine serum (FBS), 2 mM glutamine, and 1% Antibiotic Antimycotic Solution (Ab/AM) (all from Sigma-Aldrich, St Louis, MO), at 37°C in 5% CO₂/air. The cell lines were regularly tested for Mycoplasma contamination by fluorescence microscopy using DAPI staining (Molecular Probes Life Technologies, Carlsbad, CA).

Osteikoetxea X., Balogh A., Szabó-Taylor K., Németh A., Szabó T.G., Pálóczi K., Sódar B., Kittel Á., György B., Pállinger É., Matkó J, & Buzás E.I. (2015). Improved Characterization of EV Preparations Based on Protein to Lipid Ratio and Lipid Properties. PLoS ONE, 10(3), e0121184.

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Establishing Colorectal Cancer Cell Lines

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Human CRC specimen samples were procured from the Surgical Pathology Unit at the Chinese Academy of Medical Sciences Cancer Hospital (Beijing, China). Informed consent was obtained from all subjects, and this study was approved by the ethical committees of the Cancer Hospital, Chinese Academy of Medical Sciences. The clinical backgrounds for these patients are shown in Supplementary Tables S1–S3. Peripheral blood was collected from patients and centrifuged with Ficoll density gradient, following which the middle layer cells were gathered for fluorescence-activated cell sorting (FACS) analysis. Mice were housed at our Institutional Animal Care unit. All animal experiment protocols were approved by the ethical committee of the Chinese Academy of Medical Sciences, Cancer Hospital.
THP-1 (TIB-202, RRID:CVCL_0006), HCT116 (CCL-247, RRID:CVCL_0291) and DLD1 (CCL-221, RRID:CVCL_0248) cells were from ATCC. Ana-1 (EP-CL-0023, RRID:CVCL_0142) and CT26 cells (EP-CL-0071, RRID:CVCL_7254) were from Elabscience (Texas, USA). All the cells were maintained in RPMI 1640 (Bioroc™, China), supplemented with 10% heated-inactivated fetal bovine serum, 100 UI/mL penicillin, and 100 μg/mL streptomycin.

Zhao Y., Zhang W., Huo M., Wang P., Liu X., Wang Y., Li Y., Zhou Z., Xu N, & Zhu H. (2021). XBP1 regulates the protumoral function of tumor-associated macrophages in human colorectal cancer. Signal Transduction and Targeted Therapy, 6, 357.

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Immune Cell Culture and Stimulation

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THP-1 (TIB-202), RAW 264.7 (TIB-71), L929 cell lines (CCL-1), and HEK293T cells (CRL-3216) were purchased from ATCC. Human THP-1 cells were plated in RPMI 1640 medium supplemented with l-glutamine (2 mM), 10% FBS, HEPES (10 mM, pH 7.2), β-mercaptoethanol (0.05 mM), and 1% penicillin–streptomycin. THP-1 cells were treated with phorbol 12-myristate 13-acetate (PMA, 100 ng/ml, Sigma-Aldrich, Cat#: P8139) for 48 h to differentiate into macrophages. RAW 264.7 mouse macrophages and HEK293T cells were cultured in DMEM containing 10% FBS and 1% penicillin–streptomycin at 37 °C and 5% CO₂. L929 mouse fibroblast line was grown in DMEM containing 10% FBS and 1% P/S at 37 °C and 5% CO₂. Conditioned media were collected at day 3 after confluency, filtered, and stored in a −20 °C freezer. BMDMs, peritoneal macrophages, and raw264.7 cells were treated with Pam3CSK4 (100 ng/ml, Invitrogen, Cat#: tlrl-pms), LTA (100 ng/ml, Invitrogen, Cat#: vtlrl-lta), Poly(I:C) (50 μg/ml, Sigma-Aldrich, Cat#: P1530), LPS (100 ng/ml, Sigma-Aldrich, Cat#: L2630), CpG (1 μM, Invitrogen, Cat#: tlrl-1668) or IL-4 (100 ng/ml, Sigma-Aldrich, Cat#: SRP3211) for 8 h.

Wang X., Ding Y., Li R., Zhang R., Ge X., Gao R., Wang M., Huang Y., Zhang F., Zhao B., Liao W, & Du J. (2023). N6-methyladenosine of Spi2a attenuates inflammation and sepsis-associated myocardial dysfunction in mice. Nature Communications, 14, 1185.

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Cell Culture of Immune Cell Lines

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THP-1 (TIB-202), Jurkat (TIB-152), and Raji (CCL-86) cells were purchased from ATCC (Manassas, VA, USA). The cells were maintained in a RPMI1640 medium (Gibco, Life Technologies, Carlsbad, CA, USA) supplemented with 10% FBS and 1% penicillin-streptomycin (Gibco, Life Technologies, Carlsbad, CA, USA) in 5% CO₂ at 37 °C.

Besançon H., Larpin Y., Babiychuk V.S., Köffel R, & Babiychuk E.B. (2022). Engineered Liposomes Protect Immortalized Immune Cells from Cytolysins Secreted by Group A and Group G Streptococci. Cells, 11(1), 166.

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