Hsd icr cd 1 female mice

Experimental infections in mice were performed in the biosafety level 3 (BSL-3) facilities at Centro de Investigación en Sanidad Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (CISA, INIA-CSIC). Six-week-old Hsd:ICR(CD-1) female mice (Inotiv) were used. Mice were infected intraperitoneally (i.p.) with 10⁴ PFU of WNV New York 99 strain [14 (link)] (GenBank: KC407666.1) in 200 µL of Minimum Essential Medium Eagle (Corning); mock-infected animals were also inoculated i.p. with 200 µL of culture media. In the case of drug treatments, drugs or vehicle (saline buffer, 0.9% NaCl) were intraperitoneally administered at a dose of 200 mg/kg for DCA or 500 mg/kg in the case of 2-DG. Mice were daily injected (QD) from the first day of infection up to day 6 post-infection. Animals were kept with ad libitum access to food and water and were anesthetized under isoflurane and humanely killed at 7 days post-infection.

Experimental infections in mice were performed in the biosafety level 3 (BSL-3) facilities at Centro de Investigación en Sanidad Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (CISA, INIA-CSIC). Six-week-old Hsd:ICR(CD-1) female mice (Inotiv) were used. Mice were infected intraperitoneally (i.p.) with 10⁴ PFU of WNV New York 99 strain [14 (link)] (GenBank: KC407666.1) in 200 µL of Minimum Essential Medium Eagle (Corning); mock-infected animals were also inoculated i.p. with 200 µL of culture media. In the case of drug treatments, drugs or vehicle (saline buffer, 0.9% NaCl) were intraperitoneally administered at a dose of 200 mg/kg for DCA or 500 mg/kg in the case of 2-DG. Mice were daily injected (QD) from the first day of infection up to day 6 post-infection. Animals were kept with ad libitum access to food and water and were anesthetized under isoflurane and humanely killed at 7 days post-infection.