MAEC were cultured in endothelial growth medium supplemented with 5% FBS and endothelial growth supplements according to the instructions provided by Cell Biologics Inc. (Chicago, IL, USA). MAECs at passages 4–6 were used for the experiment. DMEM medium with 10% FBS was used to culture mouse monocytic WEHI78/24 cells. The cells were cultured at 37 °C in a 5% CO2/95% oxygen.
Endothelial growth supplements
Manufactured by Cell Biologics
Sourced in United States
Endothelial growth supplements are a specialized media component designed to support the growth and proliferation of endothelial cells in cell culture applications. These supplements provide a blend of growth factors, cytokines, and other essential nutrients necessary for the optimal development and maintenance of endothelial cell lines.
Automatically generated - may contain errors
Lab products found in correlation
Anti vcam 1, by BioLegend (1 mentions)
Anti tsp 1, by Santa Cruz Biotechnology (1 mentions)
Collagenase type 1a, by Merck Group (1 mentions)
Tnf α, by R&D Systems (1 mentions)
Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions)
Streptomycin, by Lonza (1 mentions)
L glutamine, by Lonza (1 mentions)
Collagenase type 1, by Worthington (1 mentions)
Anti ve cadherin, by BD (1 mentions)
2 protocols using endothelial growth supplements
1
Culturing Endothelial and Monocytic Cells
2
Isolating and Characterizing Vascular Endothelial Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Antibodies—anti-VCAM-1 (Biolegend, San Diego, CA, USA), anti-TSP-1 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-VE-cadherin and fluorochrome-conjugated anti-CD45 and anti-CD31 (BD Biosciences, San Jose, CA, USA).
Recombinant proteins—mouse IL-17 and TNF-α (R&D Systems, Minneapolis, MN, USA). Type 1A collagenase (Sigma-Aldrich St. Louis, MO, USA) and Type I collagenase (Worthington Biochemical Corp., Lakewood, NJ, USA).
Peptides—TSP-1-derived peptides 4N1K (KRFYVV MWKK, CD47-binding peptide) and CSV (CSVTCG, CD36-binding peptide), and control peptides 4NGG (KRFYGG MWKK) and ANK (ANKHYF), respectively (Bio Basic, Markham, Ontario, Canada).
Culture medium—DMEM containing 10 mM HEPES, 0.1 mM NEAA, 1 mM sodium pyruvate, 100 U/mL penicillin, 100 mg/mL streptomycin, 200 mM L-glutamine (Lonza, Basel, Switzerland), 10% fetal bovine serum (Atlanta Biologicals, Lawrenceville, GA, USA) and endothelial growth supplements (Cell Biologics Inc., Chicago, IL, USA), all purchased from Sigma–Aldrich, unless otherwise stated.
Recombinant proteins—mouse IL-17 and TNF-α (R&D Systems, Minneapolis, MN, USA). Type 1A collagenase (Sigma-Aldrich St. Louis, MO, USA) and Type I collagenase (Worthington Biochemical Corp., Lakewood, NJ, USA).
Peptides—TSP-1-derived peptides 4N1K (KRFY
Culture medium—DMEM containing 10 mM HEPES, 0.1 mM NEAA, 1 mM sodium pyruvate, 100 U/mL penicillin, 100 mg/mL streptomycin, 200 mM L-glutamine (Lonza, Basel, Switzerland), 10% fetal bovine serum (Atlanta Biologicals, Lawrenceville, GA, USA) and endothelial growth supplements (Cell Biologics Inc., Chicago, IL, USA), all purchased from Sigma–Aldrich, unless otherwise stated.
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