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2 protocols using abs against mitochondrial complexes

1

Mitochondrial Regulation in Inflammation

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All chemicals were purchased from Sigma-Aldrich (St. Louis, MO) unless specified otherwise. LPS was purchased from InvivoGen (San Diego, CA). SB302580, specific p38 inhibitor was purchased from Sigma-Aldrich. SP600125, HRP-conjugated anti-mouse and anti-rabbit IgG secondary Abs were purchased from Cell Signaling Technology (Beverly, MA) and anti-goat IgG secondary Abs were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). β-Actin Ab was purchased from Thermo Fisher Scientific (Waltham, MA). Abs against mitochondrial complexes, cytochrome c oxidase subunit I (COXI) and COXIV were purchased from MitoSciences (Eugene, OR). NRF-1, TFAM, pp38, and PGC-1α Abs were purchased from Santa Cruz Biotechnology. Superoxide dismutase (SOD)2 (manganese SOD [MnSOD]) Ab was purchased from Proteintech (Rosemont, IL). SOD1 Ab (copper-zinc SOD [Cu/ZnSOD]) was purchased from MilliporeSigma (Billerica, MA). Glucose transporter (Glut)1 Ab was purchased from Invitrogen (Carlsbad, CA).
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2

Mitochondrial Regulation in Inflammation

Check if the same lab product or an alternative is used in the 5 most similar protocols
All chemicals were purchased from Sigma-Aldrich (St. Louis, MO) unless specified otherwise. LPS was purchased from InvivoGen (San Diego, CA). SB302580, specific p38 inhibitor was purchased from Sigma-Aldrich. SP600125, HRP-conjugated anti-mouse and anti-rabbit IgG secondary Abs were purchased from Cell Signaling Technology (Beverly, MA) and anti-goat IgG secondary Abs were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). β-Actin Ab was purchased from Thermo Fisher Scientific (Waltham, MA). Abs against mitochondrial complexes, cytochrome c oxidase subunit I (COXI) and COXIV were purchased from MitoSciences (Eugene, OR). NRF-1, TFAM, pp38, and PGC-1α Abs were purchased from Santa Cruz Biotechnology. Superoxide dismutase (SOD)2 (manganese SOD [MnSOD]) Ab was purchased from Proteintech (Rosemont, IL). SOD1 Ab (copper-zinc SOD [Cu/ZnSOD]) was purchased from MilliporeSigma (Billerica, MA). Glucose transporter (Glut)1 Ab was purchased from Invitrogen (Carlsbad, CA).
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