Ab16080

Manufactured by Abcam

Sourced in United Kingdom

Ab16080 is a primary antibody that recognizes the protein of interest. It is designed for use in various laboratory techniques, such as Western blotting, immunohistochemistry, and immunocytochemistry.

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Lab products found in correlation

Ab92544, by Abcam (1 mentions) Mops sds running buffer, by Thermo Fisher Scientific (1 mentions) Mes sds, by Thermo Fisher Scientific (1 mentions) Epr19523, by Abcam (1 mentions) Ep2000y, by Abcam (1 mentions) Ab13248, by Abcam (1 mentions) Goat anti mouse irdye 800, by LI COR (1 mentions) Goat anti rabbit irdye 680, by LI COR (1 mentions) Goat anti mouse alexa 647, by Thermo Fisher Scientific (1 mentions) A11122, by Thermo Fisher Scientific (1 mentions) Mouse anti myc 9b11, by Cell Signaling Technology (1 mentions) Mouse anti β actin a5316, by Merck Group (1 mentions) Goat anti mouse hrp, by Jackson ImmunoResearch (1 mentions) Prb 101p, by Fortrea (1 mentions) Goat anti rabbit hrp, by Jackson ImmunoResearch (1 mentions)

2 protocols using ab16080

Western Blotting of Alzheimer's Proteins

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Samples were loaded onto 4–12% or 8% Bis-Tris gels using MES-SDS or MOPS-SDS running buffer (Invitrogen), transferred to nitrocellulose membranes, and probed for various proteins using standard WB. The resultant blots were detected with ECL and exposure to film. The antibodies used to detect specific antigens were, for mBACE1, EPR19523 (rabbit; Abcam); for hBACE1, D10E5 (rabbit, CST, RRID: AB_1903900); for ADAM10, MABN1123 (rabbit, RRID: AB_10972023; Abcam); for MT5-MMP, GTX128246 (rabbit; Genetex); for Meprinβ, PA5-47474 (rabbit, RRID: AB_2607364; Thermo Fisher Scientific); for PS1-N, SC-7860 (rabbit, RRID: AB_2170581; Santa-Cruz); for PS1-C, EP-2000Y (rabbit, RRID: AB_1310605; Abcam); for PS2-C, EP-1515Y (rabbit, RRID: AB_882202; Abcam); for NCT, SC-14369 (goat, RRID: AB_2282503; Santa-Cruz); for Aph-1aL, PRB-550P (rabbit, RRID: AB_291682; BioLegend): for SPP, ab16080 (rabbit, RRID: AB_302242; Abcam); for SPPL2b, PA5-42683 (rabbit, Abcam RRID: AB_2606446; Abcam); for APP-CTF, C-7 (rabbit, homemade); for APP (573–596), SIG-39180 (mouse, RRID: AB_10719734; BioLegend); for mAPP (597), 28055 (rabbit, RRID: AB_918338; IBL); for APP-CTF (asp1), 3D6 (mouse, homemade); for LRP1, ab92544 (rabbit, RRID: AB_2234877; Abcam); for APLP1, W1CT (rabbit, homemade); and for RHBDL2, 12467-1-AP (rabbit, RRID: AB_11232403; Proteintech).

Liu L., Ding L., Rovere M., Wolfe M.S, & Selkoe D.J. (2019). A cellular complex of BACE1 and γ-secretase sequentially generates Aβ from its full-length precursor. The Journal of Cell Biology, 218(2), 644-663.

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Antibody Characterization and Validation

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The following antibodies were used: rabbit anti-HA (PRB-101P; Covance), rabbit anti-SPP (ab16080; Abcam), rabbit anti–hepatitis C core (R4210; gift from J. McLauchlan, Medical Research Council–University of Glasgow Centre for Virus Research, Glasgow, Scotland, UK), mouse anti–HO-1 (ab13248; Abcam), mouse anti-myc (9B11; Cell Signaling Technology), mouse anti–β-actin (A5316; Sigma-Aldrich), rabbit anti-RAMP4/4-2 (A18; sc-85114; Santa Cruz Biotechnology, Inc.), mouse anti-calnexin (AF8) and rabbit anti-GFP (A11122; Invitrogen), rabbit anti-US2 (177–5; ER luminal domain; a gift from E. Wiertz, University Medical Center Utrecht, Utrecht, Netherlands), mouse anti–MHC-I (W6/32; ATCC), goat anti–mouse Alexa 647 (Invitrogen), goat anti–mouse IRDye 800 and goat anti–rabbit IRDye 680 (LI-COR Biosciences), and goat anti–mouse HRP and goat anti–rabbit HRP (Jackson ImmunoResearch Laboratories, Inc.).

Boname J.M., Bloor S., Wandel M.P., Nathan J.A., Antrobus R., Dingwell K.S., Thurston T.L., Smith D.L., Smith J.C., Randow F, & Lehner P.J. (2014). Cleavage by signal peptide peptidase is required for the degradation of selected tail-anchored proteins. The Journal of Cell Biology, 205(6), 847-862.

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