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Crh cre

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The Crh-Cre is a transgenic mouse line that expresses Cre recombinase under the control of the corticotropin-releasing hormone (Crh) gene promoter. This allows for cell-type-specific gene manipulation in Crh-expressing neurons.

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Lab products found in correlation

Tdtomato, by Jackson ImmunoResearch (1 mentions) Chat ires cre, by Jackson ImmunoResearch (1 mentions) Nts cre, by Jackson ImmunoResearch (1 mentions)

2 protocols using crh cre

1

Genetic Labeling of Mouse Cholinergic and Corticotropin-Releasing Neurons

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All breeding and experimental procedures were approved by the University of Louisville Institutional Animal Care and Use Committee. Cre recombinase expressing mouse lines ChAT-IRES-Cre (Jackson Labs, stock # 006410, strain B6;129S6-Chattm2(cre)Lowl/J) and Crh-Cre (MMRRC, stock # 030850-UCD, strain Tg(Crh-Cre)KN282Gsat/Mmucd) were bred to Cre-dependent reporter lines Ai9 (tdTomato; Jackson Labs, stock # 007905 B6;129S6-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J), or Ai32 (channelrhodopsin-2-eYFP; Jackson Labs, stock #012569, strain B6;129S-Gt(ROSA)26Sortm32(CAG-COP4*H134R/EYFP)Hze/J). A total of 77 mice aged P1-P120 of either sex were used in experiments and included Crh-Cre+/+ (n = 3), Crh-Cre+/− x Ai9+/− (n = 1), ChAT-Cre+/− x Ai9+/− (n = 18), and ChAT-Cre+/− x Ai32+/− (n = 55).
Sokhadze G., Campbell P.W, & Guido W. (2018). Postnatal development of cholinergic input to the thalamic reticular nucleus of the mouse. The European journal of neuroscience, 49(8), 978-989.
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2

Transgenic Mouse Strain Breeding

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All procedures were approved and conducted in accordance with the guidelines of the Institutional Animal Care and Use Committee of the University of Washington. Mice were group-housed on a 12-h light/dark cycle with ad libitum food and water. Approximately equal numbers of male and female mice were used for all experiments. Crh-Cre (Stock number 012704), Nts-Cre (Stock number 017525), Tac2-Cre (Stock number 021878), and Ai14 TdTmto (Stock number 007914) mice are available from Jackson Laboratories.
Soden M.E., Yee J.X., Cuevas B., Rastani A., Elum J, & Zweifel L.S. (2022). Distinct Encoding of Reward and Aversion by Peptidergic BNST Inputs to the VTA. Frontiers in Neural Circuits, 16, 918839.
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