Adult heart tissues were collected immediately after euthanization, and the proteins were isolated using homogenization in RIPA buffer (Sigma) with complete mini ETDA-free protease inhibitor cocktail (Roche) and PhosSTOP phosphatase inhibitor (Roche) using a Qiagen TissueRuptor. Methodologies related to protein estimation, quantitation were described previously7 . The following antibodies were used: anti- myosin-binding subunit (MBS; BioLegend), anti-phospho MBS (pMBS; Cell Signaling Technology/MBL International); anti-ROCK1 (BD Bioscience); anti-ROCK2 (BD Bioscience); anti-extracellular signal-regulated kinase (ERK; Cell Signaling Technology); anti-phospho ERK (pERK; Cell Signaling Technology); anti-protein kinase B (AKT; Cell Signaling Technology); anti-phospho AKT (pAKT; Cell Signaling Technology); anti-inhibitor of kappa B kinase (IKK; Cell Signaling Technology); anti-phospho IKK(pIKK; Cell Signaling Technology); anti-Tubulin (Sigma), anti-rabbit horseradish peroxidase (HRP; Cell Signaling Technology) and anti-mouse HRP (Cell Signaling Technology).
Anti phospho erk perk
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-phospho ERK (pERK) is a laboratory product that detects phosphorylated extracellular signal-regulated kinase (ERK) proteins. It is used to measure the activation state of the ERK signaling pathway in cells.
Automatically generated - may contain errors
Lab products found in correlation
Azd6244, by Selleck Chemicals (2 mentions)
Tissueruptor, by Qiagen (1 mentions)
Anti rock2, by BD (1 mentions)
Anti protein kinase b akt, by Cell Signaling Technology (1 mentions)
Anti rock1, by BD (1 mentions)
Anti extracellular signal regulated kinase erk, by Cell Signaling Technology (1 mentions)
Anti phospho akt p akt, by Cell Signaling Technology (1 mentions)
Phosstop phosphatase inhibitor, by Roche (1 mentions)
Ripa buffer, by Merck Group (1 mentions)
Anti tubulin, by Merck Group (1 mentions)
Gefitinib, by Selleck Chemicals (1 mentions)
Anti total erk, by Cell Signaling Technology (1 mentions)
Verteporfin, by Selleck Chemicals (1 mentions)
Anti mmp2, by GeneTex (1 mentions)
Anti bcl 2, by GeneTex (1 mentions)
Mmp2 inhibitor 1, by Santa Cruz Biotechnology (1 mentions)
Abt 199, by Active Motif (1 mentions)
Dasatinib, by LC Laboratories (1 mentions)
Anti oct4, by GeneTex (1 mentions)
Anti total akt, by Cell Signaling Technology (1 mentions)
4 protocols using anti phospho erk perk
1
Isolation and Characterization of Cardiac Proteins
2
Investigating Cellular Signaling Pathways
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Gefitinib, Verteporfin and AZD6244 were obtained from Selleckchem (Houston, TX). All other chemicals were acquired from Sigma Chemical (St. Louis, MO), unless otherwise indicated. Anti-phospho-AKT (p-AKT), Anti-AKT, anti-total ERK, and anti-phospho-ERK (p-ERK) antibodies were obtained from Cell Signaling (Danvers, MA). Anti-YAP1 was obtained from Genetex (Irvine CA). All other antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX).
3
Dasatinib and Inhibitor Combination Study
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Dasatinib was obtained from LC Laboratories (Woburn, MA, USA). MMP2 inhibitor I was acquired from Santa Cruz Biotechnology (Dallas, TX, USA). AZD6244 was acquired from Selleckchem (Houston, TX, USA). ABT-199 was obtained from ActiveBiochem (Maplewood, NJ, USA). Anti-total ERK, and anti–phospho-ERK (p-ERK) antibodies were purchased from Cell Signaling (Danvers, MA, USA). Anti-PXN antibody was obtained from NeoMarker (Fremont, CA, USA). Anti-phosphoY31-PXN (pPXN-Y31), anti-c-Myc, anti-MMP2 and anti-Bcl-2 was obtained from Genetex (Irvine, CA, USA). All other antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).
4
Evaluation of Oncogenic Pathway Inhibitors
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Cisplatin, Perifosine and AZD6244 was obtained from Selleckchem.com (Houston, TX). BBI-608 was obtained from BioVision (Mountain View, CA). All other chemicals were acquired from Sigma Chemical (St. Louis, MO) unless otherwise indicated. Anti-total AKT, anti–phospho-AKT (pAKT), anti-total ERK, anti–phospho-ERK (pERK) and anti-phospho (Ser33, Ser 37, Thr 41)-β-catenin (p-β-catenin) antibodies were obtained from Cell Signaling (Danvers, MA). Anti-PAK1, anti–phospho-PAK1 (pS144-PAK1), anti-total GSK3β, anti–phospho- GSK3β (pS9-GSK3β), anti-SOX2 and anti-OCT4 were obtained from Genetex (Irvine CA). Anti-phospho-PAK1 (pT423-PAK1) antibody was obtained from Abnova (Taiwan). All other antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX).
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