Nugenius imaging system

Manufactured by Syngene

The NuGenius imaging system is a versatile and efficient tool for capturing and analyzing images. It is designed to provide high-quality image capture and analysis capabilities across a wide range of applications. The NuGenius imaging system is capable of handling various types of samples, including gels, blots, and other biological samples, and offers a user-friendly interface for easy operation.

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Lab products found in correlation

Qubit fluorometer, by Thermo Fisher Scientific (4 mentions) Nanodrop 2000, by Thermo Fisher Scientific (4 mentions) Dsdna broad range assay kit, by Thermo Fisher Scientific (2 mentions) Pippin pulse, by Sage Science (2 mentions) Seakem gold agarose 1, by Lonza (2 mentions) Chef dna size standard ladder, by Bio-Rad (2 mentions) Quick load 1 kb extend dna ladder, by Bio-Rad (2 mentions) Femto pulse system, by Agilent Technologies (1 mentions) Genomic dna 165 kb kit, by Agilent Technologies (1 mentions)

2 protocols using nugenius imaging system

Comprehensive DNA Quantification and Quality Assessment

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DNA was quantified using a Qubit fluorometer with the dsDNA Broad Range Assay kit (Thermo Fisher Scientific) following the manufacturer’s instructions. DNA purity was evaluated using Nanodrop 2000 (Thermo Fisher Scientific) UV/Vis measurements. To determine the cell line gDNA integrity pulse-field gel electrophoresis, using the Pippin Pulse (Sage Science) was performed. For this analysis a SeaKem® GOLD Agarose 1% (Lonza) gel was prepared in 0.5× TBE buffer (Thermo Fisher Scientific). Approximately 150 ng of DNA sample was loaded together with CHEF DNA Size Standard Ladder (BIO-RAD) and Quick-Load 1 kb Extend DNA Ladder (NEB) to aid size determination. Fragments were separated using a pre-set 5–80 kb protocol. After the run, the gel was visualised using a NuGenius imaging system (Syngene). DNA integrity of the clinical samples was assessed with the Femto Pulse System using the Genomic DNA 165 kb Kit (Agilent Technologies) following the manufacturer’s protocol. The gDNA samples were stored at 4 °C.

Keraite I., Becker P., Canevazzi D., Frias-López C., Dabad M., Tonda-Hernandez R., Paramonov I., Ingham M.J., Brun-Heath I., Leno J., Abulí A., Garcia-Arumí E., Heath S.C., Gut M, & Gut I.G. (2022). A method for multiplexed full-length single-molecule sequencing of the human mitochondrial genome. Nature Communications, 13, 5902.

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DNA Quality Control for Cell Lines and Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

DNA quality control. DNA was quantified using a Qubit fluorometer with the dsDNA Broad Range Assay kit (Thermo Fisher Scientific) following the manufacturer's instructions. DNA purity was evaluated using Nanodrop 2000 (Thermo Fisher Scientific) UV/Vis measurements. To determine the cell line gDNA integrity pulse-field gel electrophoresis, using the Pippin Pulse (Sage Science) was performed. For this analysis a SeaKem® GOLD Agarose 1% (Lonza) gel was prepared in 0.5× TBE buffer (Thermo Fisher Scientific). Approximately 150 ng of DNA sample was loaded together with CHEF DNA Size Standard Ladder (BIO-RAD) and Quick-Load 1 kb
Extend DNA Ladder (NEB) to aid size determination. Fragments were separated using a pre-set 5-80 kb protocol. After the run, the gel was visualized using a NuGenius imaging system (Syngene). DNA integrity of the clinical samples was assessed with the Femto Pulse System using the Genomic DNA 165 kb Kit (Agilent Technologies) following the manufacturer's protocol. The gDNA samples were stored at 4ºC.

Keraite I., Becker P., Canevazzi D., Frías-López C., Dabad M., Tonda R., Paramonov I., Ingham M., Brun‐Heath I., Leno-Colorado J., Abulí A., Garcia-Arumí E., Heath S., Gut M., & Gut M. (2022). Novel method for multiplexed full-length single-molecule sequencing of the human mitochondrial genome.

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