Human epidermal egf and fibroblast fgf growth factors

Disposables for tissue culture were from Falcon (Steroglass, Perugia, Italy). Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham (DMEM/F-12) was purchased from Sigma-Aldrich S.p.A. (Milan, Italy) as well as penicillin/streptomycin, amphotericin B, 2′[3′]-O-[4-benzoyl benzoyl]adenosine-5′triphosphate tri[triethylammonium] salt (BzATP) and all the other chemicals, unless differently indicated. 3-[[5-[2,3-Dichlorophenyl]-1H-tetrazol-1-yl]methyl]pyridine hydro- chloride (A438079) and 3-[6-methyl-2-pyridinyl]-N-phenyl-4-[4-quinolinyl]-1H-pyrazole-1-carbo-thioamide (A8301) were ordered from Tocris Bioscience (Space Import, Milan, Italy); Transforming Growth Factor β (TGFβ) and human Epidermal (EGF) and Fibroblast (FGF) growth factors were purchased from PeproTech (SIAL, Rome, Italy).

Disposables materials for tissue culture were from Falcon (Steroglass, Perugia, Italy). Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham (DMEM/F-12) was purchased from Sigma-Aldrich S.p.A. (Milan, Italy) as well as penicillin/streptomycin, amphotericin B, and all the other chemicals, unless differently indicated. Human epidermal (EGF) and fibroblast (FGF) growth factors were purchased from PeproTech (SIAL, Rome, Italy). Protease inhibitors Mix, Immobiline Dry Strip 4–7 IPG (acrylamide gel), the Dry Strip cover fluid (98% (v/v) liquid paraffin solution), De STREAK Reydratation Solution (IEF running buffer), buffer IPG 4–7 (40% ampholin), and agarose were purchased from GE Healthcare (Uppsala, Sweden). 2.7 Disulfonicnaphthalenic acid (NDS) Acros, and porcine trypsin was provided by Thermo Fisher Scientific Geel, Belgium, and Promega Bioscience, CA, respectively. All the solutions used were prepared with Milly-Q water (Millipore, Bedford, MA, USA).