Fast Blue B salt, 2,2- diphenyl-1-picrylhydrazyl radical (DPPH, 97%), butyrylcholinesterase (BChE, from equine serum, ≥140 U/mg) and acetylcholinesterase (AChE, from Electrophorus electricus Linnæus, ≥245 U/mg), 1-naphthyl acetate, gallic acid, Trolox and solvents/reagents of analytical grade were purchased from Fluka Sigma-Aldrich, Schnelldorf, Germany. Bioluminescent marine Aliivibrio fischeri bacteria (NRRL-B11177, DSM no. 5171) were obtained from the German Collection of Microorganisms and Cell Cultures, Berlin, Germany. Ethanol, methanol and MS grade solvents (Optima LC-MS grade) methanol and acetonitrile were provided from Thermo Fisher Scientific, Schwerte, Germany. Bacillus subtilis spores (BGA, DSM 618 strain), formic acid (96%), hydrochloric acid, ethyl acetate, butanone, Folin-Ciocalteu reagent and HPTLC plates silica gel 60 F254 were obtained from Merck, Darmstadt, Germany. Thiazol blue tetrazolium bromide (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide, MTT) were purchased from Carl Roth, Karlsruhe, Germany. Bidistilled water was prepared with a Destamat Bi 18E, Heraeus, Hanau, Germany. formic acid (98%) was from J.T. Baker, Deventer, Netherlands.
1 naphthyl acetate
Manufactured by Merck Group
Sourced in United States, Poland
1-naphthyl acetate is a chemical compound used as a laboratory reagent. It is a colorless crystalline solid with a chemical formula of C₁₂H₁₀O₂. The primary function of 1-naphthyl acetate is to serve as a substrate in various enzymatic assays and analysis procedures.
Automatically generated - may contain errors
Lab products found in correlation
Fast blue b salt, by Merck Group (5 mentions)
Bovine serum albumin (bsa), by Merck Group (3 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (2 mentions)
2 naphthyl acetate, by Merck Group (2 mentions)
2 naphthyl α d glucopyranoside, by Merck Group (2 mentions)
Optima lc ms grade methanol, by Thermo Fisher Scientific (1 mentions)
Folin ciocalteu reagent, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
Ethyl acetate, by Merck Group (1 mentions)
Trolox, by Merck Group (1 mentions)
Acetylcholinesterase, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
Gallic acid, by Merck Group (1 mentions)
Hptlc plates silica gel 60 f 254, by Merck Group (1 mentions)
Butyrylcholinesterase bche, by Merck Group (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Tris hcl, by Merck Group (1 mentions)
2 2 azino bis 3 ethylbenzothiazoline 6 sulfonic acid diammonium salt, by Merck Group (1 mentions)
7 protocols using 1 naphthyl acetate
1
Antioxidant and Cholinesterase Inhibition Assays
2
Acetylcholinesterase Inhibition Assay
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Acetylcholinesterase from electric eel (EC 3.1.1.7, type V-S), bovine serum albumin (BSA), 1-naphthyl acetate, Fast Blue B salt, galantamine, Tris-HCl, dimethyl sulfoxide (DMSO), Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Phosphate buffer saline (PBS) and Penicillin/Streptomycin solution were purchased from Hyclone (Logan, Utah, USA). Trypsin-EDTA was purchased from Gibco (Waltham, MA, USA). 3-(4,5-dimetylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) was purchased from Bio Basic (Markham, Ontario, Canada). L-ascorbic acid was purchased from Calbiochem (San Diego, CA, USA). Mueller-Hinton broth was purchased from Himedia laboratories (Mumbai, MH, India). Other reagents used in extraction process were of analytical grade.
3
Phytochemical Analysis of Herbs
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Acetone (99.5%), acetic acid (99.5–99.9%), diphenylboryloxyehtylamine (NP) (≥97.0%), ethanol (96%), ethyl acetate (99.8%), 2-isopropyl-5-methyl-phenol (thymol) (≥98.5%), methanol (99.8%), o-phosphoric acid (85%), polyethylene glycol—4000 (PEG-4000), phosphate buffer, sodium hydroxide (≥98%), sulfuric acid (96–98%), and sodium acetate buffer were from POCH (Gliwice, Poland). P-Anisaldehyde (AS) (≥98%), bovine serum albumin (BSA) (≤100%), 2,2-diphenyl-1-picrylhydrazyl (DPPH) (≤100%), 3,4-dihydroxy-L-phenylalanine (L-DOPA) (>98.0%), α-glucosidase from Saccharomyces cerevisiae (≥10 units/mg protein), lipase from porcine pancreas (30–90 units/mg protein), 1-naphthyl acetate (≥98%), 2-naphthyl acetate (≤100%), 2-naphthyl α- D glucopyranoside ((≤100%), polyethylene glycol tert-octylphenyl ether (Triton X) (≤100%), rosavin (≥98.0%), salidroside (≥98.0%), p-tyrosol (98%), Fast Blue B salt (95%), tris(hydroxymethyl)aminomenthane hydrochloride (TRIS) buffer, and tyrosinase from mushroom (≥1000 unit/mg solid) were purchased from Sigma Aldrich (Poznań, Poland). Pure water was from the Millipore Q system (Millipore, Bedford, MA, USA). All reagents were of the analytical grade. Acetonitrile used in HPLC-UV/Vis-DAD quantification procedure was purchased from Romil (Cambridge, UK). TLC silica gel 60 F254 (20 × 10 cm) plates were purchased from Merck, (Darmstadt, Germany).
4
Biochemical Assay Reagents Sourcing
5
Antioxidant and Enzyme Inhibition Assays
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Acetic acid, ethanol 96%, ethyl acetate, methanol, thymol, sulfuric acid 95%, sodium acetate buffer, and phosphate buffer were from P.O.Ch. (Gliwice, Poland). Acetylcholinesterase enzyme (AChE) from Electrophorus electricus, p-anisaldehyde, bovine serum albumin (BSA), 1,1-diphenyl-2-picrylhydrazyl (DPPH), L-DOPA, Fast Blue B Salt, lipase from Porcine pancreas, 1-naphthyl acetate, 2-naphthyl acetate, 2-naphthyl α-D glucopyranoside, α-glucosidase from Saccharomyces, Natural Product—NP reagent (diphenylboryloxyethylamine), polyethylene glycol-4000, tyrosinase from mushroom and TRIS hydrochloride were purchased from Sigma Aldrich (Poznań, Poland). The MTT dye (3-(4,5-dimethyldiazol-2-yl)-2,5 diphenyltetrazolium bromide), HEPES buffer, and Triton X-100 were from Sigma Aldrich (Poznań, Poland). Agarose, Mueller-Hinton (M-H) agar, and M-H broth were purchased from Biocorp (Warsaw, Poland). The antibacterial activity was tested toward reference Gram-positive bacteria: Bacillus subtilis (ATCC 6633) was purchased from the American Type Culture Collections.
The standards of lignans were supplied by Chem-Faces Biochemical (Wuhan, China). The lignans working standards solutions were prepared in methanol.
The standards of lignans were supplied by Chem-Faces Biochemical (Wuhan, China). The lignans working standards solutions were prepared in methanol.
6
Analytical Reagents for Enzyme Assays
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Acetonitrile (99%), amitraz (> 98.0%), coumaphos (99.0%, Pestanal™ analytical standard), 7-ethoxycoumarin, tau-fluvalinate (98.7%), 1-naphthol (> 98.0%), 1-naphthyl acetate (> 98.0%), sodium dodecyl sulfate (~ 99%), Trizma base primary standard and buffer (> 99%), glutathione reductase from Baker's yeast, 1-chloro-2,4-dinitrobenzene (≥ 99%), and fast blue RR salt (95%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Acetone (HPLC grade), sodium phosphate dibasic anhydrous (98.0%), sodium phosphate monobasic monohydrate (99.5%), Triton X-100, hydrochloric acid (12.1 N), and sodium chloride (≥ 99.0%) were purchased from Fisher Scientific (Hampton, NH, USA). Coomassie brilliant blue G (electrophoresis grade) was purchased from MP Biomedical Supplies LLC (Solon, OH, USA). 7-hydroxycoumarin (99%) was purchased from Acros Organics (Pittsburg and Westchester, PA, USA).
7
Bioautographic Assay for AChE Inhibition
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Anticholinesterase activity was measured using a bioautographic assay adapted (Silveira et al., 2011) from Yang et al. (2009) (link). Acetylcholinesterase (AChE -EC 3.1.1.7, Sigma-Aldrich) was dissolved in 0.05 M Tris-hydrochloric acid buffer at pH 7.8 with 1 mg/ml bovine serum albumin (BSA, 98%, Sigma-Aldrich). The stock solution was kept at 4 °C. LECF and BECF extracts were weighed, and made up into two stock solutions of 20 mg/ml with methanol. Then different volumes of LECF and BECF stock solutions (equivalent to 600, 400, 200, 150, 100, 50 and 25 μg dry mass) were loaded on two TLC F 254 plates (10 × 10 cm, 0.2 mm thickness; Merck) and eluted in dichloromethane: methanol (9:1 v/v) for separation of compounds. The dried TLC plates were sprayed with AChE enzyme solution (1U/ml) and incubated at 37 °C for 20 minutes. For detection of the enzymes inhibitors, solutions of 1-naphthyl acetate (150 mg; Sigma-Aldrich) in ethanol 40% solution (100 ml) and Fast Blue B salt (50 mg; 95%, Sigma-Aldrich) in MilliQ water (100 ml) were prepared immediately before use. After incubation of TLC plates, the 1-naphthyl acetate and Fast Blue B salt solutions were sprayed on the plates to give a purple coloration after 1-2 minutes. The inhibition of AChE was observed from the white spots on the purple colored dye background of the TLC plates.
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