Rosa26 cag lsl enphr3.0 eyfp wpre

Manufactured by Jackson ImmunoResearch

The Rosa26-CAG-LSL-eNpHR3.0-EYFP-WPRE is a genetically engineered DNA construct designed for the controlled expression of the eNpHR3.0 (enhanced Natronomonas pharaonis halorhodopsin) protein. It features the Rosa26 promoter, a CAG (cytomegalovirus early enhancer/chicken beta-actin) promoter, a Lox-STOP-Lox (LSL) cassette, the eNpHR3.0 coding sequence, an EYFP (enhanced yellow fluorescent protein) reporter, and a WPRE (Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element) sequence. This construct allows for Cre-recombinase-dependent expression of the eNpHR3.0 protein and fluorescent labeling.

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Lab products found in correlation

Rosa26 cag lsl chr2 eyfp wpre, by Jackson ImmunoResearch (2 mentions) Gad67 gfp, by Jackson ImmunoResearch (1 mentions)

Spelling variants of this product

Rosa26-EYFP (26 citations) Rosa26-LSL-EYFP (4 citations)

2 protocols using rosa26 cag lsl enphr3.0 eyfp wpre

Transgenic Mouse Models for Neuroscience

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Wild-type mice of both sexes were used (4–6 months old, weight: 23 g ± 1.9, body length 8.2 cm ±0.3, mean ± SD). The following transgenic lines were used: Vglut2^Cre(Borgius et al., 2010 (link)), VIAAT^Cre(Hägglund et al., 2013 (link)); Rosa26-LSL-GCaMP3 (Ai38); Rosa26-CAG-LSL- eNpHR3.0-EYFP-WPRE, and Rosa26-CAG-LSL-ChR2-EYFP-WPRE (all from The Jackson Laboratories). All crosses were genotyped before experiments. Animals were housed in single cages an environmentally controlled room (22°C, 12:12 light-dark cycle). All surgical procedures and experimental manipulations were approved by the local ethical committee.

Bellardita C., Caggiano V., Leiras R., Caldeira V., Fuchs A., Bouvier J., Löw P, & Kiehn O. (2017). Spatiotemporal correlation of spinal network dynamics underlying spasms in chronic spinalized mice. eLife, 6, e23011.

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Transgenic Mouse Lines for Neuronal Tracing

Cited 1 time

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All experiments were approved by the local ethical committee. The following transgenic lines were used: Chx10∷Cre (kindly provided by S. Crone, K. Sharma, L. Zagoraiou, and T.M. Jessell, see Azim et al., (2014) (link), GlyT2-GFP (Zeilhofer et al., 2005 (link)), GAD67-GFP (Tamamaki et al., 2003 (link)), Rosa26^{FloxedSTOP-YFP} or Rosa26^{FloxedSTOP-Tdtomato}, Rosa26-CAG-LSL-eNpHR3.0-EYFP-WPRE, and Rosa26-CAG-LSL-ChR2-EYFP-WPRE (all from the Jackson Laboratories).

Bouvier J., Caggiano V., Leiras R., Caldeira V., Bellardita C., Balueva K., Fuchs A, & Kiehn O. (2015). Descending Command Neurons in the Brainstem that Halt Locomotion. Cell, 163(5), 1191-1203.

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